Heat damage to the chromosome of Escherichia coli K-12.

Applied and Environmental Microbiology
J R PellonR F Gomez

Abstract

The folded chromosome or nucleoid of Escherichia coli was analyzed by low-speed sedimentation in neutral sucrose gradients after in vivo heat treatment. Heat treatment of cultures at 50 degree C for 15, 30, and 60 min resulted in in vivo association of the nucleoids with cellular protein. Structural changes, determined by the increase in speed dependence of the nucleoids from heated cells, also occurred. These changes were most likely due to the unfolding of the typical compact nucleoid structure. The nucleoids from heated cells also had notably higher sedimentation coefficients (3,000 to 4,500S) than nucleoids from control cells (1,800S). These nucleoids did not contain greater than normal amounts of membrane phospholipids or ribonucleic acid. We propose that the protein associated with the nucleoids from heated cells causes the observed sedimentation coefficient increases.

References

Aug 1, 1977·Canadian Journal of Microbiology·A Hurst
Aug 22, 1977·Biochemical and Biophysical Research Communications·N Grecz, S Bhatarakamol
Feb 1, 1978·Journal of Bacteriology·E C Materman, A P Van Gool
Aug 1, 1978·Journal of Bacteriology·E C Materman, A P Van Gool
Jan 1, 1978·Advances in Applied Microbiology·M D PiersonS E Martin
Feb 25, 1978·Journal of Molecular Biology·D PettijohnS van Scoyk
Mar 1, 1976·Nucleic Acids Research·R M Hecht, D E Pettijohn
Nov 1, 1976·CRC Critical Reviews in Biochemistry·D E Pettijohn
May 17, 1972·Nature: New Biology·E Woodcock, G W Grigg
Jun 1, 1972·Journal of General Microbiology·S G Sedgwick, B A Bridges
Apr 29, 1969·Biochemical and Biophysical Research Communications·B A BridgesR J Munson
Sep 1, 1969·Journal of General Microbiology·B A BridgesR J Munson

❮ Previous
Next ❯

Citations

Jan 1, 1995·Journal of Basic Microbiology·J Membrillo-HernándezM C Gomez-Eichelmann
Sep 27, 2012·Archives of Microbiology·Anja BauermeisterRalf Moeller
Dec 1, 1995·Letters in Applied Microbiology·C H Lim, K P Flint
Sep 9, 2009·Journal of Applied Microbiology·E Van DerlindenJ F Van Impe
Jun 1, 1983·The Journal of Applied Bacteriology·J R Pellon
Sep 1, 1984·Applied and Environmental Microbiology·D H Bueschkens, M E Stiles
Nov 1, 1982·Applied and Environmental Microbiology·R I TomlinsR J Gray
Mar 1, 1981·Journal of Bacteriology·J R Pellon, R F Gomez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.