Heat denaturation affects the ProDer p 1 IgE reactivity and downregulates the development of the specific allergic response

The Journal of Allergy and Clinical Immunology
M MagiA Jacquet

Abstract

Modified allergens with reduced IgE-binding activity represent an elegant approach to circumvent the risk of anaphylactic reactions in allergen-specific immunotherapy. The current work investigated the effect of heat denaturation on the allergenic properties of recombinant ProDer p 1, a precursor form of the major house dust mite allergen Der p 1. The IgE reactivity was estimated by direct and competition ELISA. The immunogenicity of heat-denatured ProDer p 1 was evaluated in naive and Der p 1-allergic mice. Heat denaturation in reducing conditions drastically reduced the in vitro ProDer p 1 IgE reactivity toward human allergic sera. In naive mice, heat-denatured ProDer p 1 generated mixed T(H)1-T(H)2 responses characterized by the absence of specific IgE with concomitant rise in specific IgG2a titers and the presence of IL-5 and IFN-gamma in splenocyte cultures. In contrast, natural Der p 1 or native ProDer p 1 induced typical strict T(H)2-biased allergic responses with strong IgG1 and IgE titers, whereas spleen cells exhibited only high IL-5 secretion. Moreover, native or heat-denatured ProDer p 1 vaccinations prevented airway eosinophil infiltrations after challenge. Although native or heat-treated ProDer p 1 adjuvanted with...Continue Reading

Citations

Aug 11, 2006·Current Allergy and Asthma Reports·Enrique Fernández-CaldasMiguel Casanovas
May 16, 2012·The Journal of Biological Chemistry·Marisa K JoubertVibha Jawa
Oct 9, 2013·Critical Reviews in Immunology·Charles B SmarrStephen D Miller
Dec 24, 2005·Journal of Pharmaceutical Sciences·Vivek S PurohitSathyamangalam V Balasubramanian
Jan 21, 2009·The Journal of Allergy and Clinical Immunology·David WalgraffeAlain Jacquet
Feb 20, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D BurtinP Moingeon
Sep 12, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M HisberguesA Jacquet
Nov 15, 2005·European Journal of Immunology·Pål JohansenThomas M Kündig
Mar 9, 2006·The Journal of Allergy and Clinical Immunology·Sonia de HalleuxJean-Marie Saint-Remy
Jul 27, 2018·The Journal of Microbiology·Min Yong JwaSeung Hyun Han
Mar 30, 2017·Experimental and Therapeutic Medicine·Wei LuChenghao Zhang
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuko KikuchiHideoki Ogawa

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