Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Microbiology and Immunology
Ryota SuzukiTetsushi Yoshikawa

Abstract

A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of 350 samples (29.4%) by the original CMV LAMP procedure and 148 of 350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

References

Feb 24, 2000·Journal of Medical Virology·N TanakaT Morishima
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Nov 29, 2001·Journal of Clinical Microbiology·F GriscelliJ H Bourhis
Dec 10, 2003·Journal of Clinical Microbiology·Noritaka KubokiIkuo Igarashi
Mar 9, 2004·Journal of Clinical Microbiology·Michael BoeckhLawrence Corey
Mar 9, 2004·Journal of Clinical Microbiology·Tetsushi YoshikawaYoshizo Asano
Oct 16, 2004·Journal of Medical Virology·Souki OkamotoYoshizo Asano
Nov 6, 2004·Journal of Clinical Microbiology·Tomoko HorisakaHideki Hayashidani
Feb 8, 2005·Journal of Clinical Microbiology·Yoshihiko EnomotoYoshizo Asano
Mar 23, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Masaru IhiraTetsushi Yoshikawa
May 23, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sonali K SanghaviCharles R Rinaldo

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Citations

May 10, 2011·The Journal of Veterinary Medical Science·Manabu NemotoTomio Matsumura
Jan 22, 2011·Bioanalysis·Joonyul Kim, Christopher J Easley
Mar 1, 2014·AIDS Research and Treatment·Mohan Kumar Haleyur Giri Setty, Indira K Hewlett
Nov 5, 2016·Analytical Chemistry·Rhett L MartineauDeirdre R Meldrum
Nov 27, 2016·Biosensors & Bioelectronics·Maria Chiara Giuffrida, Giuseppe Spoto
Jul 24, 2014·Expert Review of Molecular Diagnostics·Hector David de PazCarmen Muñoz-Almagro
Oct 3, 2019·Biosensors·Faye M Walker, Kuangwen Hsieh

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