PMID: 9191897May 1, 1997Paper

Heat-induced apoptosis and p53 in cultured mammalian cells

International Journal of Radiation Biology
H NakanoK Shinohara

Abstract

Heat-induced apoptosis was studied in M10 and MOLT-4 cells by determining nuclear morphological changes, decrease in cell size, DNA degradation into fragments of about 30 kbp, and the appearance of internucleosomal DNA fragments (DNA ladders). Morphological changes in the nucleus were detected within 30 min after heat-treatment at 44 degrees C in M10 cells, but much later (> 5 h) in MOLT-4 cells. In M10 cells, 30 kbp-DNA fragments were observed even at the end of the heat-treatment and decreased 10 min later, while the DNA ladder increased at 10-30 min after heat treatment. DNA fragments of 30 kbp appeared in MOLT-4 cells at 1 h after the heat-treatment and apparently accumulated for up to 24 h. Heat-treatment increased p53 protein in MOLT-4 cells but not in M10 cells. Analysis of the DNA sequence of the p53 gene revealed that M10 cells have a heterozygous mutation in codon 173 of exon 5. These results suggest that apoptosis is induced by hyperthermia in a cell-line dependent manner, that the formation of 30 kbp-DNA fragments is a very early event in apoptosis, that DNA fragmentation into a DNA ladder occurs via the 30 kbp fragments, and that apoptosis in heat-treated M10 cells may be independent of p53.

References

Sep 30, 1992·Biochemical and Biophysical Research Communications·D BarbieriC Franceschi
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Feb 1, 1991·International Journal of Radiation Biology·B V HarmonG C Gobé
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Citations

Jan 21, 2006·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Chang-Uk LimMichael H Fox
Dec 29, 2000·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·T OhtsuboC W Song
Dec 18, 2013·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Feng LvWei You
Feb 22, 2002·Journal of Cellular Physiology·Jun GuanGeorge Iliakis
Mar 31, 2000·Experimental Cell Research·N P Singh
Apr 5, 2001·International Journal of Radiation Oncology, Biology, Physics·K KamedaH Seto

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