Heat-induced endoplasmic reticulum stress in soleus and gastrocnemius muscles and differential response to UPR pathway in rats.

Cell Stress & Chaperones
Shivani SharmaBhuvnesh Kumar

Abstract

The present study aimed to investigate the differential response of oxidative (soleus) and glycolytic (gastrocnemius) muscles to heat-induced endoplasmic reticulum (ER) stress. It was hypothesized that due to compositional and functional differences, both muscles respond differently to acute heat stress. To address this, male Sprague Dawley rats (12/group) were subjected to thermoneutral (25 °C) or heat stress (42 °C) conditions for 1 h. Soleus and gastrocnemius muscles were removed for analysis post-exposure. A significant increase in body temperature and free radical generation was observed in both the muscles following heat exposure. This further caused a significant increase in protein carbonyl content, AOPP, and lipid peroxidation in heat-stressed muscles. These changes were more pronounced in heat-stressed soleus compared to the gastrocnemius muscle. Accumulation of unfolded, denatured proteins results in ER stress, causing activation of unfolded protein response (UPR) pathway. The expressions of UPR transducers were significantly higher in soleus as compared to the gastrocnemius muscle. A significant elevation in resting intracellular calcium ion was also observed in heat-stressed soleus muscle. Overloading of cells with...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·J A Buege, S D Aust
Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Apr 22, 1974·Pflügers Archiv : European journal of physiology·P D GollnickB Saltin
May 1, 1996·Kidney International·V Witko-SarsatB Descamps-Latscha
Dec 22, 1999·Seminars in Cell & Developmental Biology·M J Gething
Dec 5, 2000·Journal of Science and Medicine in Sport·A J TattersonM A Febbraio
Feb 14, 2002·Molecular Immunology·Gennady Ermak, Kelvin J A Davies
Mar 16, 2002·Journal of Applied Physiology·Larry A SonnaCraig M Lilly
Jul 6, 2002·Critical Reviews in Oncology/hematology·Bert HildebrandtHanno Riess
Nov 20, 2002·The Journal of Clinical Investigation·Randal J Kaufman
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
Aug 26, 2004·PLoS Biology·Yong-Xu WangRonald M Evans
Mar 19, 2005·International Journal of Sports Medicine·S R McAnultyL B Gladden
Oct 4, 2005·The Journal of Clinical Investigation·Chunyan XuJohn C Reed
Dec 13, 2005·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·James R Lepock
Jan 7, 2006·Cell Death and Differentiation·J Wu, R J Kaufman
Jan 7, 2006·Cell Death and Differentiation·M Boyce, J Yuan
Apr 4, 2006·Cardiovascular Research·Aleksey V Zima, Lothar A Blatter
Jul 20, 2006·The Journal of Biological Chemistry·Daniel ShentonChris M Grant
Oct 20, 2006·Cell Calcium·Sean M Davidson, Michael R Duchen
Dec 15, 2006·World Journal of Gastroenterology : WJG·Esther E Dupont-Versteegden
Jun 15, 2007·Nature Reviews. Molecular Cell Biology·David Ron, Peter Walter
Nov 6, 2007·Antioxidants & Redox Signaling·Jyoti D Malhotra, Randal J Kaufman
Jan 25, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Joseph L Roti Roti
Jun 5, 2008·Antioxidants & Redox Signaling·Dunyaporn TrachoothamPeng Huang
Jul 30, 2008·Methods in Enzymology·Kezhong Zhang, Randal J Kaufman
Sep 2, 2008·Autophagy·Jamie M SwanlundTerry D Oberley
Oct 17, 2008·Physiological Reviews·Scott K Powers, Malcolm J Jackson
Dec 20, 2008·Journal of Applied Physiology·David G Allen
Dec 26, 2008·American Journal of Physiology. Cell Physiology·J M McClungS K Powers
Mar 14, 2009·Free Radical Biology & Medicine·Lianqin ZhangJeffrey S Shenberger
Oct 9, 2009·Muscle & Nerve·Stephen L DoddAndrew R Judge
Dec 4, 2009·American Journal of Physiology. Cell Physiology·J M McClungZ Yan
Mar 6, 2010·Journal of Applied Physiology·Melissa A WhiddenScott K Powers
Jul 27, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M A K AzadM Toyomizu

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Methods Mentioned

BETA
protein folding
MDA
electrophoresis

Software Mentioned

ImageJ
GraphPad Prism

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