PMID: 8955381Dec 1, 1996Paper

Heat induction of hsp18 gene expression in Streptomyces albus G: transcriptional and posttranscriptional regulation

Journal of Bacteriology
P Servant, P Mazodier

Abstract

In Streptomyces albus G, HSP18, a protein belonging to the small heat shock protein family, could be detected only at high temperature. The nucleotide sequence of the DNA region upstream from hsp18 contains an open reading frame (orfY) which is in the opposite orientation and 150 bp upstream. This open reading frame encodes a basic protein of 225 amino acids showing no significant similarity to any proteins found in data banks. Disruption of this gene in the S. albus chromosome generated mutants that synthesized hsp18 RNA at 30 degrees C, suggesting that orfY plays either a direct or indirect role in the transcriptional regulation of the hsp18 gene. In addition, thermally induced expression of the hsp18 gene is subject to posttranscriptional regulation. In the orfY mutant, although hsp18 RNA was synthesized at a high level at 30 degrees C, the HSP18 protein could not be detected except after heat shock. Synthesis of the HSP18 protein in the orfY mutant was also heat inducible when transcription was inhibited by rifampin. Furthermore, when wild-type cultures of S. albus were shifted from high temperature to 30 degrees C, synthesis of the gene product could no longer be detected, even though large amounts of hsp18 RNA were present.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Horwitz
Aug 5, 1992·Journal of Molecular Biology·O YarchukM Dreyfus
Apr 1, 1992·Gene·F Wright, M J Bibb
Jan 2, 1992·Nature·M J Gething, J Sambrook
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H NagaiT Yura
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A VanBogelen, F C Neidhardt
Jan 1, 1993·Annual Review of Microbiology·T YuraH Mori
Oct 17, 1994·Molecular & General Genetics : MGG·A M DuchêneP Mazodier
Jun 1, 1995·Journal of Bacteriology·P Servant, P Mazodier
Aug 1, 1993·Molecular Microbiology·B Bukau
Jan 1, 1994·Trends in Biochemical Sciences·F U HartlT Langer
May 1, 1994·Molecular Microbiology·P ServantP Mazodier
Aug 1, 1995·Molecular Microbiology·A M PugliaC J Thompson
Jan 1, 1996·Molecular Microbiology·A BrandiC L Pon
Feb 1, 1996·Molecular Microbiology·M HeckerU Völker

❮ Previous
Next ❯

Citations

Oct 28, 2016·Cell Stress & Chaperones·Wolfgang Schumann
Oct 12, 1999·Microbiology·P ServantP Mazodier
Jan 21, 2003·Mass Spectrometry Reviews·Ran Rosen, Eliora Z Ron
Apr 13, 2017·FEMS Microbiology Reviews·Davide Roncarati, Vincenzo Scarlato
Feb 23, 1999·Molecular Microbiology·C GrandvaletP Mazodier
Jun 11, 1998·Molecular Microbiology·F NarberhausH Hennecke
Nov 26, 2005·Archives of Microbiology·Sandrine BraudPhilippe Mazodier
Feb 13, 1999·Molecular Microbiology·F Narberhaus
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Franz Narberhaus
Dec 29, 1998·Journal of Bacteriology·M MünchbachF Narberhaus
Jun 26, 2003·Trends in Microbiology·Jörgen Johansson, Pascale Cossart
Sep 22, 2006·Journal of Molecular Biology·A I Jewett, J-E Shea

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.