Heat-modified citrus pectin induces apoptosis-like cell death and autophagy in HepG2 and A549 cancer cells

PloS One
Lionel LeclereCarine Michiels

Abstract

Cancer is still one of the leading causes of death worldwide, and finding new treatments remains a major challenge. Previous studies showed that modified forms of pectin, a complex polysaccharide present in the primary plant cell wall, possess anticancer properties. Nevertheless, the mechanism of action of modified pectin and the pathways involved are unclear. Here, we show that citrus pectin modified by heat treatment induced cell death in HepG2 and A549 cells. The induced cell death differs from classical apoptosis because no DNA cleavage was observed. In addition, Z-VAD-fmk, a pan-caspase inhibitor, did not influence the observed cell death in HepG2 cells but appeared to be partly protective in A549 cells, indicating that heat-modified citrus pectin might induce caspase-independent cell death. An increase in the abundance of the phosphatidylethanolamine-conjugated Light Chain 3 (LC3) protein and a decrease in p62 protein abundance were observed in both cell types when incubated in the presence of heat-modified citrus pectin. These results indicate the activation of autophagy. To our knowledge, this is the first time that autophagy has been revealed in cells incubated in the presence of a modified form of pectin. This autopha...Continue Reading

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Citations

Dec 22, 2015·Carbohydrate Polymers·Lionel LeclereCarine Michiels
Apr 14, 2016·International Journal of Molecular Sciences·Yeong-Min YooHan Sung Kim
Sep 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Yi ChanTian-Shung Wu
Jun 10, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ping-Chung KuoTian-Shung Wu
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Jun 5, 2018·Experimental Biology and Medicine·Jiyeong LeeHee-Gyoo Kang
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Mar 3, 2019·Carbohydrate Polymers·Samira Bernardino Ramos do PradoJoão Paulo Fabi
Oct 30, 2021·Critical Reviews in Food Science and Nutrition·Bing ZhengQiang Yu

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Methods Mentioned

BETA
ELISA
ubiquitination
confocal microscopy
of

Software Mentioned

Microplate manager
Odyssey

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