Heat Resistance Mediated by pLM58 Plasmid-Borne ClpL in Listeria monocytogenes

MSphere
Anna PöntinenHannu Korkeala

Abstract

Listeria monocytogenes is one of the most heat-resistant non-spore-forming food-borne pathogens and poses a notable risk to food safety, particularly when mild heat treatments are used in food processing and preparation. While general heat stress properties and response mechanisms of L. monocytogenes have been described, accessory mechanisms providing particular L. monocytogenes strains with the advantage of enhanced heat resistance are unknown. Here, we report plasmid-mediated heat resistance of L. monocytogenes for the first time. This resistance is mediated by the ATP-dependent protease ClpL. We tested the survival of two wild-type L. monocytogenes strains-both of serotype 1/2c, sequence type ST9, and high sequence identity-at high temperatures and compared their genome composition in order to identify genetic mechanisms involved in their heat survival phenotype. L. monocytogenes AT3E was more heat resistant (0.0 CFU/ml log10 reduction) than strain AL4E (1.4 CFU/ml log10 reduction) after heating at 55°C for 40 min. A prominent difference in the genome compositions of the two strains was a 58-kb plasmid (pLM58) harbored by the heat-resistant AT3E strain, suggesting plasmid-mediated heat resistance. Indeed, plasmid curing resu...Continue Reading

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Citations

Nov 22, 2020·International Journal of Molecular Sciences·Cora ChmielowskaDariusz Bartosik
Mar 30, 2021·Frontiers in Microbiology·Stephan Schmitz-EsserBienvenido W Cortes
Apr 7, 2019·International Journal of Food Microbiology·Annabel L NaditzStephan Schmitz-Esser
Jun 12, 2021·International Journal of Food Microbiology·Pan MaoChangyun Ye
Aug 15, 2020·Biochemistry·Biswanath Jana, Indranil Biswas
Sep 10, 2021·FEMS Microbiology Letters·Justin M Anast, Stephan Schmitz-Esser

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Datasets Mentioned

BETA
CP023754
CP023752
CP023753
PRJNA412588

Methods Mentioned

BETA
PCR
protein folding
PCA

Software Mentioned

SnapGene Viewer
BRIG
PHASTER
SEED Viewer
grofit
BLAST
R
FGENESB
BLAST ring image generator
RAST

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