Heat shock, but not the reactive state per se, induces increased expression of the small stress proteins hsp25 and alpha B-crystallin in glial cells in vitro

Neuroreport
M BrzyskaG J Bosman

Abstract

The stress proteins hsp25 and alpha B-crystallin are found in increased concentrations in reactive astrocytes of brains undergoing neurodegeneration. In order to characterize this reaction, we investigated the expression of hsp25 and alpha B-crystallin during growth and after stress (heat shock) in glial cells in vitro. In primary rat brain cultures, hsp25 was present in actively dividing astrocytes that were positive for glial fibrillary acidic protein. alpha B-crystallin was found predominantly in oligodendrocytes. Heat shock resulted in increased concentrations of hsp25 and alpha B-crystallin in astrocytes, without any detectable changes in intracellular localization, as detectable with confocal laser microscopy. These results indicate that a neurodegeneration-related increase of the small stress proteins in astrocytes in independent of gliosis per se, and may be a disease-related event.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Horwitz

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Citations

Aug 10, 2000·Neuropathology and Applied Neurobiology·M W Head, J E Goldman
May 10, 2000·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·T ShinoharaL T Chylack
Mar 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serena CarraAngelo Poletti
Apr 16, 2010·The Journal of Comparative Neurology·C A DuffinC L Armstrong
Jul 18, 2006·Journal of Neuroimmunology·Chunhe WangArthur A Vandenbark
Oct 31, 2009·BMC Microbiology·Raphaela Castro GeorgSuely Lopes Gomes
May 8, 2003·Acta Neuropathologica·Masayuki MinamiHiroshi Mori

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