Heat shock factor 1 inhibits nuclear factor-kappaB nuclear binding activity during endotoxin tolerance and heat shock

Journal of Critical Care
Mingchen SongJohn A Kellum

Abstract

Sepsis, endotoxin tolerance, and heat shock (HS) all display down-regulation of innate immunity. We hypothesize that HS factor 1 (HSF-1) induces competitive inhibition of nuclear factor-kappaB (NF-kappaB)-induced signal transduction in both endotoxin tolerance and HS. We compared endotoxin tolerance and HS in RAW 264.7 cells. We transfected cells with an HS protein 70 (HSP70) plasmid to test whether HSP70 is the mediator of HS-induced NF-kappaB inhibition. We studied the effects of endotoxin stimulation and HS, both separately and together, on "wild-type" cells, cells transfected with the HSP70 plasmid, and cells transfected with vehicle. Heat shock protein 70 plasmid-transfected cells had increased HSP70 expression and demonstrated decreased nitric oxide (NO) release and inducible NO synthase messenger RNA expression in response to endotoxin compared with wild-type and empty plasmid-transfected cells. Heat shock completely abolished subsequent NO and inducible NO synthase messenger RNA expression in wild-type cells. Heat shock factor 1 reached maximum expression 60 to 90 minutes after HS. Heat shock protein 70-transfected cells still displayed endotoxin-induced NF-kappaB nuclear binding, whereas endotoxin tolerance, HS, and ex...Continue Reading

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Citations

May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karthikeyan KrishnamurthyGovindasamy Ilangovan
Dec 31, 2010·Shock·Samuel KobbaAnne A Knowlton
Sep 29, 2012·Clinical & Developmental Immunology·Efrossini Briassouli, George Briassoulis
Mar 14, 2012·Toxicology Mechanisms and Methods·Ekambaram PadminiLavanya Srinivasan
Mar 13, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Miguel Moreno-GarcíaHumberto Lanz-Mendoza
Mar 30, 2017·Menopause : the Journal of the North American Menopause Society·Hsin-Hsueh ShenYen-Mei Lee
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ziyan LuKhalilah L Gates
Aug 31, 2013·Journal of Leukocyte Biology·Sujatha Muralidharan, Pranoti Mandrekar
Jul 16, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sujatha MuralidharanPranoti Mandrekar
Oct 12, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alain P GobertKeith T Wilson

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