PMID: 11328606May 1, 2001Paper

Heat shock factor 2 is involved in the upregulation of alphaB-crystallin by high extracellular potassium

Journal of Biochemistry
C SadamitsuA Iwaki

Abstract

alphaB-Crystallin, a member of the small heat shock protein (HSP) family, accumulates in reactive astrocytes in a variety of pathological conditions. We previously reported the upregulation of alphaB-crystallin in response to high extracellular potassium concentration. In the present study, we investigated the regulatory mechanism of alphaB-crystallin expression by KCl. When human glioma U-251MG cells were exposed to continuous KCl treatment, induction of alphaB-crystallin mRNA was observed after 8 h and persisted for a few days. Functional promoter analysis using deletion and mutation constructs revealed that the proximal heat shock element (HSE-P), which contributes to heat shock induction in HeLa cells, is essential for transcriptional activation of the alphaB-crystallin gene by KCl in U-251MG cells. Gel mobility shift and antibody supershift assays showed that KCl induces the HSE-binding activity of heat shock factor (HSF) 2, while heat shock induces that of HSF1. This is the first demonstration that HSF2 can be activated by KCl and is involved in the upregulation of alphaB-crystallin gene expression in glial cells.

Citations

Jul 3, 1998·International Journal of Biological Macromolecules·W W de JongJ A Leunissen
Jan 17, 2008·European Journal of Human Genetics : EJHG·Tiziana BachettiIsabella Ceccherini
Jul 4, 2012·The International Journal of Biochemistry & Cell Biology·Aurélie de ThonelValérie Mezger
Apr 25, 2007·Traffic·Philippe NizardDenis Michel
Jan 29, 2016·Pharmacology & Therapeutics·Dmitry MalinVincent L Cryns

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