Heat shock factor gains access to the yeast HSC82 promoter independently of other sequence-specific factors and antagonizes nucleosomal repression of basal and induced transcription.

Molecular and Cellular Biology
A M ErkineD S Gross

Abstract

Transcription in eukaryotic cells occurs in the context of chromatin. Binding of sequence-specific regulatory factors must contend with the presence of nucleosomes for establishment of a committed preinitiation complex. Here we demonstrate that the high-affinity binding site for heat shock transcription factor (HSF) is occupied independently of other cis-regulatory elements and is critically required for preventing nucleosomal assembly over the yeast HSC82 core promoter under both noninducing (basal) and inducing conditions. Chromosomal mutation of this sequence, termed HSE1, erases the HSF footprint and abolishes both transcription and in vivo occupancy of the TATA box. Moreover, it dramatically reduces promoter chromatin accessibility to DNase I and TaqI, as the nuclease-hypersensitive region is replaced by a localized nucleosome. By comparison, in situ mutagenesis of two other promoter elements engaged in stable protein-DNA interactions in vivo, the GRF2/REB1 site and the TATA box, despite reducing transcription three- to fivefold, does not compromise the nucleosome-free state of the promoter. The GRF2-binding factor appears to facilitate the binding of proteins to both HSE1 and TATA, as these sequences, while still occupied...Continue Reading

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Citations

Sep 18, 1997·The Journal of Experimental Medicine·F C MillsE E Max
May 25, 2015·Cell Stress & Chaperones·Levi M SmithAlexandre M Erkine
Jan 28, 2006·FEMS Microbiology Reviews·James P BurnieRuth C Matthews
Apr 16, 2013·Nucleic Acids Research·Daniela StrenkertMichael Schroda
Feb 7, 2007·Plant & Cell Physiology·Yuichi KodamaKo Kato
Jul 23, 2004·Anti-cancer Drugs·Jason Beliakoff, Luke Whitesell
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·An Jansen, Kevin J Verstrepen
May 18, 2016·Molecular and Cellular Biology·Jayamani AnandhakumarDavid S Gross
Apr 30, 2005·The EMBO Journal·Arindam DasguptaDavid T Auble
Jul 19, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jakob H Waterborg
Oct 6, 2000·Physical Review Letters·K Bourke ArnvigK Sneppen
Dec 25, 2002·The Journal of Biological Chemistry·Alexander M Erkine, David S Gross
Mar 15, 2003·The Journal of Biological Chemistry·Michaela AngermayrWolfhard Bandlow
Jun 25, 1998·Molecular and Cellular Biology·K L Wang, J R Warner
Jan 24, 2008·Molecular and Cellular Biology·Sushma Shivaswamy, Vishwanath R Iyer
Aug 11, 2000·Molecular and Cellular Biology·C B VenturiD S Gross
Feb 18, 1999·Molecular and Cellular Biology·A M ErkineD S Gross
Sep 18, 2009·The Journal of Biological Chemistry·Selena B Kremer, David S Gross
Jul 24, 2012·Genome Research·Gil HornungNaama Barkai

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