Heat Shock Factor Genes of Tall Fescue and Perennial Ryegrass in Response to Temperature Stress by RNA-Seq Analysis

Frontiers in Plant Science
Yan WangXin-Rong Ma

Abstract

Heat shock factors (Hsfs) are important regulators of stress-response in plants. However, our understanding of Hsf genes and their responses to temperature stresses in two Pooideae cool-season grasses, Festuca arundinacea, and Lolium perenne, is limited. Here we conducted comparative transcriptome analyses of plant leaves exposed to heat or cold stress for 10 h. Approximately, 30% and 25% of the genes expressed in the two species showed significant changes under heat and cold stress, respectively, including subsets of Hsfs and their target genes. We uncovered 74 Hsfs in F. arundinacea and 52 Hsfs in L. perenne, and categorized these genes into three subfamilies, HsfA, HsfB, and HsfC based on protein sequence homology to known Hsf members in model organisms. The Hsfs showed a strong response to heat and/or cold stress. The expression of HsfAs was elevated under heat stress, especially in class HsfA2, which exhibited the most dramatic responses. HsfBs were upregulated by the both temperature conditions, and HsfCs mainly showed an increase in expression under cold stress. The target genes of Hsfs, such as heat shock protein (HSP), ascorbate peroxidase (APX), inositol-3-phosphate synthase (IPS), and galactinol synthase (GOLS1), sho...Continue Reading

References

Oct 23, 1997·Genome Research·S Audic, J M Claverie
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 14, 2002·The Journal of Biological Chemistry·Concepción AlmogueraJuan Jordano
May 8, 2004·Trends in Plant Science·Wangxia WangArie Altman
Dec 18, 2004·Plant Molecular Biology·Eva Czarnecka-VernerWilliam B Gurley
Nov 3, 2006·Plant Physiology and Biochemistry : PPB·C Zhang, C L Guy
Dec 8, 2006·The Journal of Biological Chemistry·Sanjeev K BaniwalLutz Nover
Sep 11, 2007·Trends in Plant Science·Pascal von Koskull-DöringLutz Nover
Feb 12, 2008·Biochemical and Biophysical Research Communications·Takumi YoshidaKazuko Yamaguchi-Shinozaki
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jul 23, 2008·Plant Biotechnology Journal·John FernandesVirginia Walbot
Aug 23, 2008·Journal of Proteomics·Anna Maria TimperioLello Zolla
Jan 22, 2009·Plant, Cell & Environment·Joanna TrippKlaus-Dieter Scharf
Mar 28, 2009·Plant & Cell Physiology·Miho Ikeda, Masaru Ohme-Takagi
Sep 2, 2009·Current Genomics·Silvia R Ramirez, Chhandak Basu
Sep 21, 2010·Biochemical and Biophysical Research Communications·Huan ChenChae Oh Lim
Dec 1, 2010·Proceedings of the National Academy of Sciences of the United States of America·Raúl CarrancoJuan Jordano
Jan 19, 2011·Plant, Cell & Environment·Hsiang-Chin LiuYee-Yung Charng
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Oct 29, 2011·Biochimica Et Biophysica Acta·Klaus-Dieter ScharfLutz Nover
Feb 1, 2012·Journal of Experimental Botany·Julia Krasensky, Claudia Jonak
Apr 20, 2012·BMC Genomics·Bruno StuderTorben Asp
Aug 28, 2012·Plant Science : an International Journal of Experimental Plant Biology·Ching-Hui YehYee-Yung Charng
Dec 21, 2012·Journal of Experimental Botany·Elizabeth R Waters
Mar 8, 2013·International Journal of Molecular Sciences·Kenji Miura, Tsuyoshi Furumoto
May 7, 2013·International Journal of Molecular Sciences·Mirza HasanuzzamanMasayuki Fujita
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Jul 14, 2020·International Journal of Genomics·Aref AlshameriSaleh Alansi

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Datasets Mentioned

BETA
SRP059410
SRP059405

Methods Mentioned

BETA
RNA-Seq

Software Mentioned

ClustalW
filter
MEGA
Premier
blastx
TermFinder
GO
HemI

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