Heat-shock-induced refolding entails rapid degradation of bsrG toxin mRNA by RNases Y and J1

Microbiology
Natalie Jahn, Sabine Brantl

Abstract

Gene regulation accomplished by alternative folding of an mRNA is a widely used mechanism. Classical examples are the various transcriptional attenuation mechanisms that employ, for example, leader peptide translation, or binding of a modified protein, an uncharged tRNA or an antisense RNA to the 5' untranslated region of an mRNA. With the discovery of transcriptional and translational riboswitches, it became clear that small metabolites or even metal ions can also alter RNA secondary structures and, hence, gene expression. In addition, biophysical factors like temperature can affect RNA folding, as exemplified by RNA thermometers. We have investigated in detail the type I toxin-antitoxin system bsrG/SR4 from Bacillus subtilis. The antitoxin SR4 is a cis-encoded regulatory RNA that neutralizes BsrG toxin action. SR4 prevents toxin expression by promoting degradation of the toxin mRNA and inhibiting its translation. In addition, upon temperature shock the amount of toxin mRNA decreases significantly. Here, we demonstrate that heat shock induces a refolding in the central region of the toxin mRNA that makes it more accessible to degradation by RNases Y and J1. Furthermore, we show that BsrG might play a role at the onset of stati...Continue Reading

References

Apr 1, 1996·Journal of Bacteriology·W Wang, D H Bechhofer
Sep 17, 2005·Molecular Microbiology·Andreas LichtSabine Brantl
Jan 28, 2006·FEMS Microbiology Reviews·Franz NarberhausSaheli Chowdhury
Mar 23, 2007·Current Opinion in Microbiology·Kenn Gerdes, E Gerhart H Wagner
Mar 27, 2007·Current Opinion in Microbiology·Rebecca L CoppinsEduardo A Groisman
Jun 20, 2007·Nucleic Acids Research·Nadja HeidrichSabine Brantl
Mar 3, 2009·Current Opinion in Microbiology·Michael D Dambach, Wade C Winkler
Mar 21, 2009·Annual Review of Biochemistry·Adam Roth, Ronald R Breaker
Sep 26, 2009·The EMBO Journal·Karen ShahbabianHarald Putzer
Nov 26, 2009·Genes & Development·Gal NechooshtanShoshy Altuvia
Feb 5, 2010·Molecular Cell·Anna Maria GiuliodoriCynthia L Pon
May 7, 2010·Molecular Microbiology·Matthias GimpelSabine Brantl
Nov 26, 2010·Cold Spring Harbor Perspectives in Biology·Ronald R Breaker
Jan 11, 2012·Molecular Microbiology·Natalie JahnSabine Brantl
Mar 17, 2012·Nature Reviews. Microbiology·Jens Kortmann, Franz Narberhaus
Oct 13, 2012·RNA Biology·Sylvain DurandSabine Brantl
Jan 18, 2013·RNA Biology·Sabine Brantl
Jan 18, 2013·PloS One·Soumaya LaalamiHarald Putzer
Feb 15, 2013·PLoS Genetics·Ragnhild Weel-SneveMagnar Bjørås
Aug 24, 2013·Nucleic Acids Research·Natalie Jahn, Sabine Brantl
Sep 26, 2013·Cellular and Molecular Life Sciences : CMLS·Soumaya LaalamiHarald Putzer
Mar 1, 2014·RNA Biology·Sabine Brantl, Reinhold Brückner
Oct 4, 2014·Frontiers in Cellular and Infection Microbiology·Francesco Righetti, Franz Narberhaus
Mar 27, 2015·FEMS Microbiology Reviews·Sabine Brantl, Natalie Jahn
Nov 14, 2015·The Journal of Biological Chemistry·Christin MeißnerSabine Brantl

❮ Previous
Next ❯

Citations

Sep 11, 2018·Biotechnology and Bioengineering·Sen YangZhen Kang
May 9, 2019·Toxins·Olga Soutourina
May 12, 2019·Toxins·Sabine Brantl, Peter Müller
Aug 28, 2020·Frontiers in Molecular Biosciences·Inam Ul HaqSabine Brantl

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.