Heat shock induction of apoptosis in promastigotes of the unicellular organism Leishmania (Leishmania) amazonensis

Journal of Cellular Physiology
M E MoreiraMarcello André Barcinski

Abstract

Apoptosis and/or programmed cell death have been described in examples ranging from fungi to man as gene-regulated processes with roles in cell and tissue physiopathology. These processes require the operation of an intercellular communicating network able to deliver alternative signals for cells with different fates and is thus considered a prerogative of multicellular organisms. Promastigotes from Leishmania (Leishmania) amazonensis, when shifted from their optimal in vitro growth temperature (22 degrees C) to the temperature of the mammalian host (37 degrees C), die by a calcium-modulated mechanism. More parasites die in the presence of this ion than in its absence, as detected by a colorimetric assay based on the activity of mitochondrial and cytoplasmic dehydrogenases which measures cell death, independently of the process by which it occurs. A heat shock, unable to induce detectable parasite death (34 degrees C for 1 h), is able to significantly raise the concentration of intracellular free calcium in these cells. Heat-shocked parasites present ultrastructural and molecular features characteristic of cells dying by apoptosis. Morphological changes, observed only in the presence of calcium, are mainly nuclear. Cytoplasmic ...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y SchleinG Messer
Dec 1, 1992·The Journal of Cell Biology·C KosekiQ al-Awqati
Apr 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L E Gerschenson, R J Rotello
Apr 1, 1992·Molecular and Biochemical Parasitology·E KardamiR R Fandrich
Jan 24, 1991·Journal of Immunological Methods·A FacchinettiG Biasi
Feb 1, 1990·Molecular and Biochemical Parasitology·G BenaimJ A Urbina
May 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J McConkeyS Orrenius
Feb 15, 1989·Archives of Biochemistry and Biophysics·D J McConkeyS Orrenius
Jan 1, 1986·Annual Review of Genetics·D Kaiser
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Feb 1, 1988·Experimental Parasitology·R M SmejkalJ G Olenick
Jul 1, 1988·Molecular and Biochemical Parasitology·W O Rogers, D F Wirth
Dec 1, 1994·International Journal for Parasitology·K Vickerman
Feb 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B F Trump, I K Berezesky
Oct 1, 1994·The Journal of Experimental Medicine·S N MorenoR Docampo
Nov 15, 1994·European Journal of Biochemistry·D ShiokawaS Tanuma
Sep 1, 1994·International Journal for Parasitology·H HeckerW Schlimme
Jul 1, 1994·Molecular and Biochemical Parasitology·M A YakubuF Kierszenbaum
Sep 1, 1994·Experimental Parasitology·I Maudlin, S C Welburn
Jan 1, 1994·Parasitology·D L SacksP F Pimenta
Mar 11, 1994·Cell·D L VauxA Strasser
Jun 1, 1993·The American Journal of Tropical Medicine and Hygiene·R Charlab, J M Ribeiro
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L Vaux
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L M SchwartzB A Osborne
Jun 1, 1994·Trends in Cell Biology·G I Evan
Jan 1, 1994·Parasitology Today·M A Barcinski, M E Costa-Moreira
Feb 1, 1990·Parasitology Today·T SeebackD Lawson
Jun 29, 1990·European Journal of Protistology·R MilderE Freymuller

❮ Previous
Next ❯

Citations

Sep 29, 2007·Cell Death and Differentiation·A AmbitJ C Mottram
Jun 16, 2011·Photomedicine and Laser Surgery·Dennis SongMauricio S Baptista
Jul 23, 2008·Antimicrobial Agents and Chemotherapy·Juan Fernando AlzateAntonio Jiménez-Ruiz
Jun 16, 2001·Antimicrobial Agents and Chemotherapy·D SerenoJ L Lemesre
Feb 26, 2004·Antimicrobial Agents and Chemotherapy·Caroline ParisJaqueline Bréard
Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Navin K Verma, Chinmoy S Dey
Feb 20, 2010·Journal of Biomedicine & Biotechnology·Santiago Martínez-CalvilloElisa E Figueroa-Angulo
Dec 22, 2010·Malaria Journal·Beth K Mutai, John N Waitumbi
May 30, 2009·PloS One·João Luiz Mendes WanderleyMarcello André Barcinski
Dec 17, 2011·PLoS Pathogens·Sarah E ReeceAndy Gardner
Nov 13, 2002·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·P KopecekE Weigl
Apr 21, 2012·Journal of Parasitology Research·Joao Luiz Mendes WanderleyMarcello Barcinski
Nov 18, 2010·Parasites & Vectors·Laura C PollittSarah E Reece
Dec 4, 2010·Parasites & Vectors·Carsten Gk LüderGer van Zandbergen
Mar 3, 2017·Cell Death & Disease·Mauricio S PeñaBeatriz S Stolf
May 9, 2000·Memórias do Instituto Oswaldo Cruz·A L BerthoS G Coutinho
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M A Barcinski, G A DosReis
Mar 30, 2004·Anais Da Academia Brasileira De Ciências·Maria Elisabete C Moreira, Marcello A Barcinski
Mar 17, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A L BerthoS G Coutinho
May 4, 2005·Memórias do Instituto Oswaldo Cruz·Poliana DeolindoRenato A DaMatta
Dec 1, 2001·Current Biology : CB·J M de Freitas BalancoM A Barcinski
Dec 22, 2006·Protein Expression and Purification·Mithu GuhaUday Bandyopadhyay
Apr 7, 2005·Molecular and Biochemical Parasitology·Gaganmeet SinghChinmoy S Dey
Feb 23, 2008·Biochimica Et Biophysica Acta·Marcel Deponte
Jan 23, 2002·Cell Death and Differentiation·N LeeH L Nakhasi
May 23, 2003·Cell Death and Differentiation·T Kobayashi, H Endoh
Oct 5, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Oleg E AkilovTayyaba Hasan
Mar 11, 2005·Parasitology Research·Ana María Fernández PresasKaethe Willms
Aug 16, 2005·Cellular Microbiology·Eric GalianaLaure Boudier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis