Heat shock protein 27 downstream of P38-PI3K/Akt signaling antagonizes melatonin-induced apoptosis of SGC-7901 gastric cancer cells

Cancer Cell International
Wenjie DengJun Du

Abstract

Despite the fact that melatonin treatment shows some promise in gastric cancer, the molecular mechanisms of gastric cancer cells in response to melatonin remains to be determined. The SGC-7901 gastric cancer cells were treated with different concentrations of melatonin for 24 and 48 h. Cell viability was determined by MTT assay, Hoechst 33258 staining and FACS analysis were used to detect apoptotic cells. The contents and activation of apoptosis-related proteins HSP27, Akt and P38 were evaluated by immunoblotting analysis. Then we treated SGC-7901 cells with HSP27-specific siRNA, PI3K inhibitor LY294002 or P38 inhibitor SB203580 to investigate the role of HSP27, Akt and P38 in the anti-apoptotic response of SGC-7901 cells to melatonin. Melatonin suppressed cell viability and stimulated apoptosis of gastric cancer SGC-7901 cells dose-dependently. Mechanistically, the observed apoptosis was accompanied by the melatonin-induced phosphorylation of HSP27. HSP27-specific siRNA transfection effectively reduced HSP27 phosphorylation and augmented melatonin-induced apoptosis, indicating that HSP27 is resistant to melatonin-induced apoptosis. Moreover, melatonin increased PI3K/Akt activation, LY294002 abrogated HSP27 activation and promo...Continue Reading

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Citations

Mar 11, 2018·Journal of Cellular and Molecular Medicine·Wenjie DengJun Du
Sep 8, 2018·Journal of Cellular Physiology·Reyhaneh Moradi MarjanehSeyed Hamid Aghaee-Bakhtiari
Nov 25, 2018·Journal of Cellular Physiology·Atena SoleimaniSeyed Mahdi Hassanian
Mar 4, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jun-Te HsuTa-Sen Yeh
Jan 7, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jinzhong YuBo Li
May 19, 2019·Digestive Diseases and Sciences·Fei MaSuxia Luo
Oct 15, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ruiting ShanBin Liu
Jul 18, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Bingwei ZhangKe Tan

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
FACS

Software Mentioned

Image J
SPSS
Quantity One

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