Heat shock protein 27 promotes cell cycle progression by down-regulating E2F transcription factor 4 and retinoblastoma family protein p130

The Journal of Biological Chemistry
Ah-Mee ParkOsamu Yoshie

Abstract

Heat shock protein 27 (HSP27) protects cells under stress. Here, we demonstrate that HSP27 also promotes cell cycle progression of MRC-5 human lung fibroblast cells. Serum starvation for 24 h induced G1 arrest in these cells, and upon serum refeeding, the cells initiated cell cycle progression accompanied by an increase in HSP27 protein levels. HSP27 levels peaked at 12 h, and transcriptional up-regulation of six G2/M-related genes (CCNA2, CCNB1, CCNB2, CDC25C, CDCA3, and CDK1) peaked at 24-48 h. siRNA-mediated HSP27 silencing in proliferating MRC-5 cells induced G2 arrest coinciding with down-regulation of these six genes. Of note, the promoters of all of these genes have the cell cycle-dependent element and/or the cell cycle gene-homology region. These promoter regions are known to be bound by the E2F family proteins (E2F-1 to E2F-8) and retinoblastoma (RB) family proteins (RB1, p107, and p130), among which E2F-4 and p130 were strongly up-regulated in HSP27-knockdown cells. E2F-4 or p130 knockdown concomitant with the HSP27 knockdown rescued MRC-5 cells from G2 arrest and up-regulated the six cell cycle genes. Moreover, we observed cellular senescence in MRC-5 cells on day 3 after the HSP27 knockdown, as evidenced by increase...Continue Reading

References

Jul 11, 1970·Nature·J P JacobsJ P Baille
Nov 21, 1998·Veterinary Pathology·K A Schafer
Feb 1, 2000·Journal of Cellular Physiology·T Scholzen, J Gerdes
Jun 23, 2001·Oncogene·F Hans, S Dimitrov
Jun 20, 2003·Cell Proliferation·Katrien VermeulenZwi N Berneman
Aug 5, 2003·Molecular and Cellular Biology·Arnaud ParcellierCarmen Garrido
Feb 10, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Xuwan LiuBalvin H L Chua
May 15, 2004·DNA and Cell Biology·Michael Bukrinsky, Yuqi Zhao
May 4, 2005·Biochemical and Biophysical Research Communications·Andrei V Gudkov, Elena A Komarova
Aug 5, 2005·Nature·Manuel ColladoManuel Serrano
Dec 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gary K Schwartz, Manish A Shah
Apr 28, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Arnaud ParcellierCarmen Garrido
Aug 26, 2006·Mechanisms of Ageing and Development·Ximing ZhouDonald A Jurivich
May 18, 2007·Cell Cycle·Dragos PlescaAlexandru Almasan
Aug 2, 2007·Nature Reviews. Molecular Cell Biology·Judith Campisi, Fabrizio d'Adda di Fagagna
Dec 20, 2007·Cancer Research·Cornelia O'Callaghan-SunolMichael Y Sherman
Aug 23, 2008·Nature Reviews. Molecular Cell Biology·Sander van den Heuvel, Nicholas J Dyson
Aug 30, 2008·Cell·Valery KrizhanovskyScott W Lowe
Aug 7, 2009·The Journal of Biological Chemistry·Meyer J FriedmanXiao-Jiang Li
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Arturo V OrjaloJudith Campisi
Feb 16, 2011·The Journal of Cell Biology·Francis Rodier, Judith Campisi
Oct 13, 2011·The Journal of Pathology·Gareth H Williams, Kai Stoeber
Mar 28, 2012·The International Journal of Biochemistry & Cell Biology·C GarridoH H Kampinga
Jun 12, 2012·The International Journal of Biochemistry & Cell Biology·G WettsteinPh Bonniaud
Jun 20, 2012·The International Journal of Biochemistry & Cell Biology·Elisabeth S ChristiansIvor J Benjamin
Nov 13, 2012·Annual Review of Physiology·Judith Campisi
Nov 29, 2012·Cell Cycle·Olga V LeontievaMikhail V Blagosklonny
Mar 5, 2013·The Journal of Clinical Investigation·Tamara TchkoniaJames L Kirkland
Oct 22, 2013·Current Drug Targets·Julie AcunzoPalma Rocchi
Dec 9, 2014·Cell Cycle·Mikhail V Blagosklonny
Jan 8, 2015·Cell Cycle·Véronique Gire, Vjekoslav Dulic
Aug 10, 2015·Methods in Molecular Biology·Ingrid Thurlings, Alain de Bruin
Oct 19, 2016·Molecular Biology Reports·Menderes Yusuf TerziBulent Gogebakan
Dec 26, 2016·Trends in Pharmacological Sciences·Jianming WuShousong Cao
Mar 11, 2017·Trends in Molecular Medicine·Alexandros G GeorgakilasWilliam M Bonner

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Sep 14, 2019·International Journal of Molecular Sciences·Benjamin J LangStuart K Calderwood
Jul 2, 2019·Molecular Medicine Reports·Kwangkho KimHyun-Soo Cho
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