Heat shock protein 27 protects against aminolevulinic acid-mediated photodynamic therapy-induced apoptosis and necrosis in human breast cancer cells

Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer
Sarah A ZieglerStephen W Carper

Abstract

This study utilized two breast cancer cell lines differing only in their expression of heat shock protein 27 (hsp27). The DB46 cell line was engineered to express high constitutive levels of hsp27, while the DC4 cell line expresses normal low levels of hsp27. The cells were incubated in 1 mM aminolevlinic acid (ALA) 4 hr prior to light exposures (635 nm) ranging from 1 to 20 J/cm2. Both cell lines displayed a dose response to photodynamic therapy (PDT) as assayed by clonogenic survival. LD50s of 2.68 and 1.27 J/cm2 were observed for DB46 and DC4 cells respectively. ALA-PDT-induced resistance to both apoptosis and necrosis in the DB46 cell line was found from TUNEL assays and fluorescence microscopy studies using propidium iodide and Hoechst staining.

Citations

Mar 21, 2013·The Journal of Surgical Research·Brandon NokesJulie E Lang
Dec 15, 2010·Trends in Immunology·Francisco J Quintana, Irun R Cohen
Feb 11, 2014·Laser Therapy·Carlo FornainiJean-Paul Rocca
Jan 27, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Matías Exequiel RodríguezViviana Alicia Rivarola

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis