Heat shock protein 70 is required for tabtoxinine-β-lactam-induced cell death in Nicotiana benthamiana

Journal of Plant Physiology
Makoto ItoAkinori Kiba

Abstract

Tabtoxinine-β-lactam (TβL), a non-specific bacterial toxin, is produced by Pseudomonas syringae pv. tabaci, the causal agent of tobacco wildfire disease. TβL causes death of plant cells through the inhibition of glutamine synthetase, which leads to an abnormal accumulation of ammonium ions and the characteristic necrotic wildfire lesions. To better understand the mechanisms involved in TβL-induced cell death, we studied its regulation in Nicotiana benthamiana. TβL-induced lesions, similar to those in controls, could be observed in SGT1-, RAR1- and Hsp90-silenced plants. In contrast, Hsp70-silenced plants showed suppression of lesion formation. Expression of hin1, a marker gene for the hypersensitive response (HR), which is a characteristic of programmed cell death in plants, was strongly induced in controls by TβL treatment but only slightly in Hsp70-silenced plants. However, in these TβL-treated Hsp70-silenced plants, the amount of ammonium ions was considerably increased. Furthermore, the silencing of Hsp70 also suppressed l-methionine sulfoximine-induced cell death and hin1 expression and caused the over-accumulation of ammonium ions. When inoculated directly with P. syringae pv. tabaci, Hsp70-silenced plants showed only red...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 1, 1995·The Plant Journal : for Cell and Molecular Biology·D C BaulcombeS Santa Cruz
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·M B DickmanR French
Feb 16, 2002·Science·Cristina AzevedoPaul Schulze-Lefert
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Feb 7, 2004·Cellular Microbiology·Jean T Greenberg, Nan Yao
Mar 12, 2004·Annual Review of Phytopathology·D G Gilchrist
Apr 1, 1983·Plant Physiology·M D ThomasR D Durbin
May 9, 2006·Cell·Bernd BukauArthur Horwich
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl

❮ Previous
Next ❯

Citations

May 16, 2020·Journal of Experimental Botany·Akinori KibaYasufumi Hikichi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis