Heat shock protein 90 acts in brassinosteroid signaling through interaction with BES1/BZR1 transcription factor

Journal of Plant Physiology
Tomoaki ShigetaShigehisa Okamoto

Abstract

Brassinosteroids (BRs), a class of phytohormones, control various physiological and developmental processes in plants. Two highly homologous transcription factors, brassinosteroid insensitive 1-EMS-SUPRESSOR 1 (BES1) and brassinazole resistant 1 (BZR1), act downstream of BR signaling to control several thousands of putative target genes. We reported previously that BES1 forms a complex with a molecular chaperone: heat shock protein 90 (HSP90). This study demonstrates that the amino-terminal and central parts of BES1 are responsible for its physical interaction with HSP90.3 in vitro. Additionally, we present evidence that BZR1 is a novel HSP90 partner aside from two BR signaling components previously identified as its clients: BES1 and brassinosteroid insensitive 2 (BIN2). Furthermore, geldanamycin, an inhibitor of ATPase activity in HSP90, caused BES1 hyperphosphorylation and disrupted the expression of BR-responsive genes. Considered together, our results imply that HSP90 takes a part in BR-mediated gene expression through complex formation with two major transcription factors.

References

Oct 16, 2001·Cell Stress & Chaperones·P Krishna, G Gloor
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Steven D. Clouse, Jenneth M. Sasse
Jan 18, 2005·Current Opinion in Plant Biology·Todd A Sangster, Christine Queitsch

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Citations

Apr 29, 2016·The Plant Journal : for Cell and Molecular Biology·Theoni MargaritopoulouPolydefkis Hatzopoulos
Apr 16, 2020·Journal of Experimental Botany·Tereza TicháJozef Šamaj
Jun 19, 2019·FEBS Letters·Martin di Donato, Markus Geisler
Jul 24, 2020·International Journal of Molecular Sciences·Victor P Bulgakov, Tatiana V Avramenko
Mar 2, 2021·Frontiers in Plant Science·Ning LiWoe Yeon Kim
Apr 4, 2021·International Journal of Molecular Sciences·Aishwarya Kothari, Jennifer Lachowiec
Jun 4, 2021·Journal of Experimental Botany·Christian Danve M Castroverde, Damaris Dina
Jun 5, 2021·The New Phytologist·Despina SamakovliPolydefkis Hatzopoulos
Nov 1, 2021·FEMS Yeast Research·Julia L Crunden, Stephanie Diezmann

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