Heat shock protein-containing exosomes in mid-trimester amniotic fluids

Journal of Reproductive Immunology
Alexzander AseaSteven S Witkin

Abstract

Exosomes are multivesicular bodies formed by inverse membrane budding into the lumen of an endocytic compartment. Fusion with the plasma membrane leads to their release into the external milieu. The incorporation of heat shock proteins into exosomes has been associated with immune regulatory activity. We have examined whether heat shock protein-containing exosomes are present in mid-trimester amniotic fluid. Exosomes were isolated from mid-trimester amniotic fluids by sequential low-speed and high-speed centrifugation followed by sucrose density gradient centrifugation. Biochemical characterization included floatation pattern in sucrose gradients, acetylcholinesterase (AChE) activity and Western blot analysis for exosome-containing proteins. Exosomes were present in each of 23 amniotic fluids tested. They banded at a density of 1.17g/ml in sucrose gradients, were positive for AChE activity and contained tubulin, the inducible 72kDa heat shock protein, Hsp72 and the constitutively expressed heat shock protein, Hsc73; they were negative for calnexin. Exosome concentrations correlated positively with the number of pregnancies. Heat shock protein-containing exosomes are constituents of mid-trimester amniotic fluids and may contribu...Continue Reading

References

Aug 15, 1998·American Journal of Obstetrics and Gynecology·R GomezS M Berry
May 8, 2000·The Prostate·F G KravetsS A Khan
Mar 18, 2003·The Journal of Biological Chemistry·Ariel SavinaMaria I Colombo
Feb 27, 2004·Cell Stress & Chaperones·Jay CampisiMonika Fleshner
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
Jul 29, 2005·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M Fleshner, J D Johnson
Jun 8, 2007·Biochemical and Biophysical Research Communications·Jörg M BangenStefanie B Flohé
Aug 19, 2007·Kidney International·S KellerP Altevogt
Oct 5, 2007·Journal of Reproductive Immunology·Devrim SezenSteven S Witkin

❮ Previous
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Citations

Oct 13, 2009·Cell Stress & Chaperones·Attila MolvarecJános Rigó
Oct 7, 2010·Human Reproduction Update·L Hui, D W Bianchi
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Feb 26, 2014·Stem Cells and Development·Peter J QuesenberryGiovanni Camussi
May 28, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jessica L WilliamsCaroline C Whitacre
Jun 24, 2014·Cellular & Molecular Immunology·Dionne TannettaJennifer Southcombe
Dec 22, 2010·Seminars in Immunopathology·Nathalie Chaput, Clotilde Théry
Nov 28, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Tomi T KanninenSteven S Witkin
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Jul 6, 2010·Journal of Proteomics·Suresh MathivananRichard J Simpson
Aug 13, 2009·Journal of Reproductive Immunology·Devrim SezenSteven S Witkin
Jun 30, 2015·Molecular Aspects of Medicine·Keon MahmoudiCostas Hadjipanayis
Aug 26, 2009·American Journal of Reproductive Immunology : AJRI·Uma PerniSteven S Witkin
Mar 24, 2011·Acta Obstetricia Et Gynecologica Scandinavica·Shih-Yin HuangChia-Li Yu
Dec 12, 2012·American Journal of Reproductive Immunology : AJRI·Geeta BhatRamkumar Menon
Nov 9, 2010·BJOG : an International Journal of Obstetrics and Gynaecology·S S WitkinD Skupski
Feb 20, 2016·Expert Review of Molecular Diagnostics·Oscar A Tovar-CamargoAjay Goel
Mar 26, 2013·Proteomics·Masayuki Shimoda, Rama Khokha
Mar 25, 2010·American Journal of Reproductive Immunology : AJRI·Lucia Mincheva-Nilsson, Vladimir Baranov
Mar 25, 2016·Scientific Reports·Lucia PaoliniPaolo Bergese
Jun 3, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Sukhbir KaurDavid D Roberts
Jan 11, 2014·Seminars in Immunology·Lida A Beninson, Monika Fleshner
Nov 13, 2014·Bioinformatics·Dae-Kyum KimYong Song Gho
Oct 23, 2014·Cellular and Molecular Life Sciences : CMLS·Lindsey T BrintonKimberly A Kelly
Nov 22, 2014·The Journal of Clinical Investigation·Zongdi FengStanley M Lemon
Jun 9, 2016·Human Molecular Genetics·Elod KortvelyMarius Ueffing
Jun 22, 2016·Molecular Diagnosis & Therapy·Lindsay N StrotmanMark W Linder
Jul 8, 2016·Journal of Human Genetics·Takashi KinoshitaFei-Fei Liu
Aug 20, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Shuangpeng CaiJun Li
Jan 10, 2017·ACS Nano·James P K ArmstrongMolly M Stevens
Dec 8, 2016·Journal of Cellular Physiology·Maria M BarrecaFabiana Geraci
Feb 28, 2018·High-throughput·Stefania BortoluzziLara Mussolin
Aug 15, 2017·BioTechniques·Maja KosanovićMiroslava Janković
Mar 6, 2018·Journal of Zhejiang University. Science. B·Zhen-Yu YaoLing Gao
Jan 13, 2018·Journal of Cellular Physiology·Enrica UrciuoliBarbara Peruzzi
Oct 19, 2017·Cancer Biomarkers : Section a of Disease Markers·Chengfei ZhaoQicai Liu
Dec 22, 2017·Reviews in the Neurosciences·Baichuan SunYu Wang
Feb 2, 2018·Endocrine Reviews·Carlos SimonFelipe Vilella
Oct 18, 2013·Radiology and Oncology·Eva OgorevcPeter Veranic
Oct 26, 2018·Biotechnology Journal·Kalimuthu KalishwaralalKi Soo Park
Oct 9, 2009·American Journal of Reproductive Immunology : AJRI·Catherine HerwaySteven S Witkin
Sep 8, 2010·Journal of Pediatric Gastroenterology and Nutrition·Erna SzikszBeáta Szebeni
Jan 24, 2018·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Ji Eun SongWang Jae Lee

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