Heat shock proteins are differentially expressed in brain and spinal cord: implications for multiple sclerosis

Clinical and Experimental Immunology
R P GorterS Amor

Abstract

Multiple sclerosis (MS) is a chronic neurodegenerative disease characterized by demyelination, inflammation and neurodegeneration throughout the central nervous system. Although spinal cord pathology is an important factor contributing to disease progression, few studies have examined MS lesions in the spinal cord and how they differ from brain lesions. In this study we have compared brain and spinal cord white (WM) and grey (GM) matter from MS and control tissues, focusing on small heat shock proteins (HSPB) and HSP16.2. Western blotting was used to examine protein levels of HSPB1, HSPB5, HSPB6, HSPB8 and HSP16.2 in brain and spinal cord from MS and age-matched non-neurological controls. Immunohistochemistry was used to examine expression of the HSPs in MS spinal cord lesions and controls. Expression levels were quantified using ImageJ. Western blotting revealed significantly higher levels of HSPB1, HSPB6 and HSPB8 in MS and control spinal cord compared to brain tissues. No differences in HSPB5 and HSP16.2 protein levels were observed, although HSPB5 protein levels were higher in brain WM versus GM. In MS spinal cord lesions, increased HSPB1 and HSPB5 expression was observed in astrocytes, and increased neuronal expression of ...Continue Reading

References

Apr 1, 1991·Journal of Neuroscience Research·E LecainB Pessac
Oct 23, 1997·Journal of Neuroimmunology·J J BajramovićJ M van Noort
Nov 17, 1999·The European Journal of Neuroscience·L SchnellV H Perry
Jan 13, 2000·Neuropathology and Applied Neurobiology·P GanterM M Esiri
Apr 17, 2002·Lancet·Alastair Compston, Alasdair Coles
Jun 3, 2004·The Journal of Comparative Neurology·Patrick G SullivanStephen W Scheff
Dec 16, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Catherine M DreizaAlyssa Panitch
May 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mieke F RoelofsTimothy R D J Radstake
Feb 6, 2007·European Journal of Cell Biology·Szabolcs BellyeiFerenc Gallyas
Mar 16, 2007·Neuron Glia Biology·Jason G Emsley, Jeffrey D Macklis
Nov 13, 2007·The Journal of Biological Chemistry·Virginia FonteChristopher D Link
Jun 3, 2008·Cellular and Molecular Life Sciences : CMLS·A NairS D Miller
Nov 7, 2008·Journal of Neurotrauma·Peter E BatchelorDavid W Howells
Jan 9, 2009·Human Molecular Genetics·Tracy L HagemannAlbee Messing
Jan 28, 2009·Brain Pathology·Christopher P GilmoreNikos Evangelou
Dec 17, 2009·Acta Neuropathologica·Michael V Sofroniew, Harry V Vinters
Jun 11, 2010·Journal of Neuropathology and Experimental Neurology·Johannes M van NoortSandra Amor
Aug 6, 2010·Annals of Neurology·Géraldine AndrodiasSerge Nataf
Sep 22, 2010·Brain : a Journal of Neurology·Stephan BramowHenning Laursen
Oct 23, 2010·Molecular Cell·Klaus RichterJohannes Buchner
Jan 12, 2011·Progress in Neurobiology·J Middeldorp, E M Hol
Jan 21, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alexander V PanovHerbert L Bonkovsky
Jan 12, 2012·The International Journal of Biochemistry & Cell Biology·Johannes M van NoortSandra Amor
May 19, 2012·Developmental Cell·Brian T KeadyGregory J Pazour
Jun 12, 2012·The International Journal of Biochemistry & Cell Biology·G WettsteinPh Bonniaud
Jun 23, 2012·The International Journal of Biochemistry & Cell Biology·Harm H Kampinga, Carmen Garrido
May 24, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·André-Patrick Arrigo, Benjamin Gibert
Jun 28, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dominique ThuringerCarmen Garrido
Nov 22, 2014·Cold Spring Harbor Perspectives in Biology·Omer Ali BayraktarDavid H Rowitch
Jun 7, 2015·Biochimica Et Biophysica Acta·Jennifer E Dulle, Patrice E Fort
Sep 30, 2015·Biochimica Et Biophysica Acta·Raman BakthisaranCh Mohan Rao
Dec 24, 2015·Acta Neuropathologica Communications·Laura A N PeferoenSandra Amor
May 4, 2016·Tissue Barriers·Imola WilhelmIstván A Krizbai
Feb 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Hedwich F KuipersLawrence Steinman
Apr 13, 2017·Brain Pathology·Natalia PetrovaKlaus Schmierer
Jul 21, 2017·Neuron·Margaret M McCarthy
Apr 13, 2018·Brain : a Journal of Neurology·Benjamin N ConradSeth A Smith

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Citations

Apr 8, 2020·Cell Stress & Chaperones·Miranda P Collier, Justin L P Benesch
Jul 11, 2020·Cellular and Molecular Life Sciences : CMLS·Inge L WerkmanWia Baron
Jun 27, 2021·Journal of Neuroimmunology·Shweta JakhmolaHem Chandra Jha

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Datasets Mentioned

BETA
AB96837

Methods Mentioned

BETA
Protein Assay
electrophoresis

Software Mentioned

Scion Image
GraphPad Prism
ImageJ
Odyssey
Scion

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