PMID: 8587806Jan 1, 1996Paper

Heat shock response of Trichinella spiralis and T. pseudospiralis

Parasitology
R C Ko, L Fan

Abstract

Heat shock proteins (HSPs) were documented for the first time in both somatic extracts and excretory/secretory (ES) products of the infective-stage larvae of Trichinella spiralis and T. pseudospiralis. Larvae recovered from muscles of infected mice were heat shocked at 37, 40, 43 and 45 degrees C in RPMI 1640 medium containing L(-)[35S]methionine. Somatic extracts and ES products of heat-shocked worms were then analysed by SDS-PAGE, autoradiography and laser densitometry. Prominent bands of HSPs were observed at 43 degrees C which is the optimal heat shock temperature. The major HSPs in somatic extracts of T. spiralis were 20, 47, 50, 70, 80 and 86 kDa. When the temperature was increased from 37 to 43 degrees C, the greatest increase in absorbance was observed in HSPs 70 and 86. In vitro translation of mRNA in a nuclease-treated rabbit reticulocyte lysate system showed an increase in the synthesis of the 80 kDa protein. This suggests that the production of HSP 80 is regulated at the transcriptional level. The major HSPs in the ES products were 11, 45, 53 and 64 kDa. In T. pseudospiralis, the major HSPs in the somatic extracts were 20, 26, 31, 50, 53, 70, 80 and 86 kDa, and in the ES products, 11, 35, 37, 41 and 64 kDa.

References

Jun 1, 1988·Molecular and Biochemical Parasitology·R HedstromG Newport
Aug 1, 1989·Molecular and Biochemical Parasitology·K S JohnsonJ S Cordingley
Dec 1, 1988·Veterinary Parasitology·H R GambleK D Murrell
Apr 28, 1988·Nature·H Pelham
Nov 1, 1988·Parasitology Today·G NewportN Agabian
Aug 1, 1992·Parasitology Today·B Maresca, L Carratù

❮ Previous
Next ❯

Citations

Jun 8, 2001·Parasitology·L Vargas-ParadaJ P Laclette
Jun 1, 1997·Journal of Helminthology·R K Leung, R C Ko
Nov 7, 2008·Parasitology Research·Isao NaganoYuzo Takahashi
Nov 1, 2002·The Journal of Experimental Zoology·Javier MartinezFilomena Rodriguez-Caabeiro
Mar 28, 2003·The Journal of Parasitology·Isao NaganoYuzo Takahashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.