Abstract
Heat shock proteins (HSPs) were documented for the first time in both somatic extracts and excretory/secretory (ES) products of the infective-stage larvae of Trichinella spiralis and T. pseudospiralis. Larvae recovered from muscles of infected mice were heat shocked at 37, 40, 43 and 45 degrees C in RPMI 1640 medium containing L(-)[35S]methionine. Somatic extracts and ES products of heat-shocked worms were then analysed by SDS-PAGE, autoradiography and laser densitometry. Prominent bands of HSPs were observed at 43 degrees C which is the optimal heat shock temperature. The major HSPs in somatic extracts of T. spiralis were 20, 47, 50, 70, 80 and 86 kDa. When the temperature was increased from 37 to 43 degrees C, the greatest increase in absorbance was observed in HSPs 70 and 86. In vitro translation of mRNA in a nuclease-treated rabbit reticulocyte lysate system showed an increase in the synthesis of the 80 kDa protein. This suggests that the production of HSP 80 is regulated at the transcriptional level. The major HSPs in the ES products were 11, 45, 53 and 64 kDa. In T. pseudospiralis, the major HSPs in the somatic extracts were 20, 26, 31, 50, 53, 70, 80 and 86 kDa, and in the ES products, 11, 35, 37, 41 and 64 kDa.
References
Sep 1, 1992·Molecular and Biochemical Parasitology·S OrtnerM Duchêne
Feb 1, 1991·Parasitology·D L LeeM H Yeung
Feb 1, 1991·The Biochemical Journal·P J HigginsM P Ryan
Jan 1, 1991·Parasitology Research·R C Ko, M H Yeung
Jun 1, 1988·Molecular and Biochemical Parasitology·R HedstromG Newport
Aug 1, 1989·Molecular and Biochemical Parasitology·K S JohnsonJ S Cordingley
Mar 15, 1989·Molecular and Biochemical Parasitology·N M RothsteinT V Rajan
Dec 1, 1988·Veterinary Parasitology·H R GambleK D Murrell
Apr 28, 1988·Nature·H Pelham
Jul 1, 1984·Veterinary Immunology and Immunopathology·H R Gamble, C E Graham
Feb 1, 1994·Parasitology·R C KoH Compton
Nov 1, 1988·Parasitology Today·G NewportN Agabian
Aug 1, 1992·Parasitology Today·B Maresca, L Carratù
Citations
Mar 12, 2004·International Journal for Parasitology·Isao NaganoYuzo Takahashi
Oct 4, 2011·Molecular & Cellular Proteomics : MCP·Hanns SoblikNorbert W Brattig
Jun 1, 2000·Folia Parasitologica·F BozićE Duraković
Apr 9, 2014·Parasitology Research·Yurong YangYan Liu
Jun 8, 2001·Parasitology·L Vargas-ParadaJ P Laclette
Oct 2, 2001·Parasitology·C H MakR C Ko
Jul 6, 2005·Parasitology Research·J Martínez, F Rodríguez-Caabeiro
Feb 3, 2007·Parasitology Research·Z WuY Takahashi
Jun 1, 1997·Journal of Helminthology·R K Leung, R C Ko
Nov 7, 2008·Parasitology Research·Isao NaganoYuzo Takahashi
Nov 1, 2002·The Journal of Experimental Zoology·Javier MartinezFilomena Rodriguez-Caabeiro
Feb 3, 2009·Acta Tropica·Shaohua WangShijuan Cui
May 12, 2015·Folia Parasitologica·Justyna BienBozena Moskwa
Nov 22, 2017·Parasites & Vectors·Yang WangXiaolei Liu
Mar 28, 2003·The Journal of Parasitology·Isao NaganoYuzo Takahashi
Oct 8, 2004·Journal of Helminthology·J MartinezF Rodriguez-Caabeiro
May 23, 2002·Journal of Helminthology·I NaganoY Takahashi
Nov 14, 2018·Parasites & Vectors·Sylwia GrzelakJustyna Bień
Mar 30, 1999·European Journal of Biochemistry·C H Mak, R C Ko