Heat shock transcription factor HSF1 regulates the expression of the Huntingtin-interacting protein HYPK

Biochimica Et Biophysica Acta
Hiroshi SakuraiEi Kawahara

Abstract

The Huntingtin-interacting protein HYPK possesses chaperone-like activity. We hypothesized that the expression of HYPK could be regulated by heat shock factor HSF1, a transcriptional regulator of chaperone genes. HYPK expression in HeLa cells was assessed by RT-PCR and Western blot analysis. In vivo binding of HSF1 to the HYPK promoter was analyzed by chromatin immunoprecipitation assays. The requirement for HYPK in heat-shocked cells was examined using HYPK-knockdown cells. Levels of HYPK mRNA were slightly increased by heat treatment; however, the levels decreased in HSF1-silenced cells. The HYPK promoter was bound by HSF1 in a heat-inducible manner; however, its core promoter activity was notably suppressed upon heat shock. When cells were exposed to heat shock, silencing HYPK caused a decrease in cell viability. HYPK is a novel target gene of HSF1. HSF1 maintains HYPK expression in heat-shocked cells. The maintenance of HYPK expression by HSF1 is necessary for the survival of cells under thermal stress conditions.

References

Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B J WuR I Morimoto
Aug 13, 1998·Human Molecular Genetics·P W FaberM E MacDonald
Oct 5, 2001·Molecular and Cellular Biology·A MathewR I Morimoto
Mar 10, 2004·Cell·Andrew D BasehoarB Franklin Pugh
Jun 15, 2005·Annual Review of Biochemistry·Martin Schröder, Randal J Kaufman
Jul 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hendrik OttoSabine Rospert
Mar 4, 2008·Molecular Cell·Peter D MarinerJames A Goodrich
Mar 21, 2009·Annual Review of Biochemistry·Evan T PowersWilliam E Balch
Aug 18, 2009·Developmental Biology·Tamar Juven-Gershon, James T Kadonaga
Jul 16, 2010·Nature Reviews. Molecular Cell Biology·Malin AkerfeltLea Sistonen
Oct 15, 2010·The FEBS Journal·Mitsuaki Fujimoto, Akira Nakai
Oct 23, 2010·Molecular Cell·Keith A SpriggsAnne E Willis
Jan 20, 2011·The Nurse Practitioner·Angela CumminsJulianne S Collins
Dec 2, 2011·Nature Reviews. Drug Discovery·Daniel W NeefDennis J Thiele
Jun 19, 2013·Trends in Biochemical Sciences·John Labbadia, Richard I Morimoto

❮ Previous
Next ❯

Citations

May 13, 2014·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yoshiyuki Suzuki
Mar 31, 2015·FEBS Letters·Yukio IshikawaHiroshi Sakurai
Aug 4, 2015·Brain Research·Irfan A Qureshi, Mark F Mehler
Aug 15, 2014·Journal of Biosciences·Swasti RaychaudhuriNitai P Bhattacharyya
Nov 28, 2021·Autophagy·Debasish Kumar Ghosh, Akash Ranjan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Ying LiuHua-li Zhang
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Zhong-Nu Ma, Yu Cao
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
E Shun-meiXian-zhong Xiao
© 2021 Meta ULC. All rights reserved