Heat-stabilized glycosphingolipid films for biosensing applications

Langmuir : the ACS Journal of Surfaces and Colloids
Rory StineCara-Lynne Schengrund

Abstract

We have investigated a means of producing thin, oriented lipid monolayers which are stable under repeated washing and which may be useful in biosensing or surface-coating applications. Phosphatidylcholine and the glycosphingolipid GM1 were used as representative lipids for this work. Initially, a mixed self-assembled monolayer of octanethiol and hexadecanethiol was produced on a gold surface. This hydrophobic monolayer was then brought into contact with a thin lipid film that had been assembled at the liquid/air interface of a solution, allowing the lipid to deposit on the gold surface through hydrophobic interactions. The lipid layer was then heated to cause intermingling of the fatty acid and alkanethiol chains and cooled to form a highly stable film which withstood repeated rinsing and solution exposure. Presence and stability of the film were confirmed via ellipsometry, Fourier transform infrared spectroscopy, and quartz crystal microbalance (QCM), with an average overall film thickness of approximately 3.5 nm. This method was then utilized to produce GM1 layers on gold-coated QCM crystals for affinity sensing trials with cholera toxin. For these sensing elements, the lower detection limit of cholera toxin was found to be a...Continue Reading

Citations

Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·K KaladharC P Sharma
May 15, 2013·ACS Applied Materials & Interfaces·Takehiro NagatsukaEiichi Tamiya
Oct 28, 2008·Journal of Colloid and Interface Science·Olga Santos, Thomas Arnebrant
Sep 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Chang-Ying XueKun-Lin Yang
Apr 30, 2005·Analytical Chemistry·Rory StineCara-Lynne Schengrund
Nov 16, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Rory StinePaul S Weiss

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