PMID: 8589919Oct 1, 1995Paper

Heat-stable antigen is expressed by murine keratinocytes and delivers costimulatory signals in T-cell activation

Experimental Dermatology
G ErdmannA H Enk

Abstract

Heat-stable antigen (HSA), expressed by various antigen-presenting cells (APC), has been described as a costimulatory molecule for CD4+ T cells. Recently, we observed that HSA also serves as an important costimulatory molecule on epidermal Langerhans cells (LC). During these studies, low levels of HSA staining were also detected on normal murine keratinocytes (KC). To investigate whether HSA also is involved in T-cell activation by KC, normal murine KC or the spontaneously transformed KC cell-line PAM 212 were treated with PDB or PMA to induce HSA-expression. FACS analyses showed induction of HSA expression on normal murine KC, as well as PAM 212 cells. In functional assays PDB or PMA-treated normal or transformed KC were far more potent inducers of primary allogeneic T-cell responses than untreated KC. Addition of anti-HSA-specific mAb 20C9 specifically inhibited the costimulatory activity of KC, an effect that was even more pronounced when CTLA-4Ig was added to the cultures. Cleavage of HSA on KC surfaces by a phosphoinositol-specific phospholipase C (PI-PLC) also significantly inhibited the costimulatory capacity of KC for naive CD4+ T cells. In aggregate, our data indicate that expression of HSA on activated KC contributes ...Continue Reading

References

Oct 1, 1992·The Journal of Experimental Medicine·C P LarsenR P Lowry
Feb 11, 1992·The Journal of Experimental Medicine·Y LiuC A Janeway
Nov 11, 1992·European Journal of Immunology·Y LiuP S Linley
May 1, 1992·The Journal of Experimental Medicine·K InabaG Schuler
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A H Enk, S I Katz
Jun 30, 1989·Cell·R H Schwartz

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Citations

Oct 20, 1998·International Journal of Immunopharmacology·D J ClementsK L McCoy
Oct 3, 1998·Experimental Dermatology·P RedondoE Quintanilla
Jan 11, 2008·The Journal of Investigative Dermatology·Susanne StutteIrmgard Förster
Jun 1, 1997·Immunology and Cell Biology·K Ni, H C O'Neill
Apr 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huiming HonJoshy Jacob

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