Heat-stable chloroplastic Cu/Zn superoxide dismutase in Chenopodium murale

Biochemical and Biophysical Research Communications
Renu Khanna-Chopra, S Sabarinath

Abstract

Chenopodium murale is a weed species having wide adaptation to different climatic regimes and experiences a temperature range of 5-45 degrees C during its life span. Higher temperatures may result in heat stress, which induces higher ROS production leading to oxidative stress in the plant. Superoxide dismutase enzyme (SOD, EC.1.15.1.1) is ubiquitous, being widely distributed among O(2)(-) consuming organisms and is the first line of defense against oxidative stress. In this study, we have characterized the thermostability of the SOD isozymes from C. murale in vitro. The leaf protein extracts, thylakoidal and stromal fractions were subjected to elevated temperatures ranging from 50 degrees C to boiling and analyzed for activity and isoform pattern of SOD. Out of six SOD isoforms, SOD V showed stability even after boiling the extract for 10min. Under high temperature treatment (>60 degrees C) there was an appearance of a new SOD band with higher electrophoretic mobility. The inhibitor studies and subcellular analysis revealed that the SOD V isoform was a chloroplastic Cu/Zn SOD. The stromal Cu/Zn SOD (SOD V) was more stable than the co-migrating thylakoidal isozyme at 80 degrees C and boiling for 10min. Hence, we report an unusua...Continue Reading

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Citations

Jul 1, 2009·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sabarinath SundaramRenu Khanna-Chopra
Apr 24, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Vimal Kumar SemwalRenu Khanna-Chopra
Nov 20, 2008·Journal of Integrative Plant Biology·Bingru Huang, Chenping Xu
Sep 1, 2006·Plant Science : an International Journal of Experimental Plant Biology·Tingting QinLinfang Du
Jul 19, 2011·Biochemical and Biophysical Research Communications·Renu Khanna-ChopraVimal Kumar Semwal
Oct 6, 2010·Critical Reviews in Biotechnology·Amit BafanaParamvir S Ahuja
Oct 1, 2011·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Renu Khanna-Chopra, Vimal Kumar Semwal
Jan 10, 2019·Environmental Science and Pollution Research International·Ali Ahsan BajwaBhagirath Singh Chauhan

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