Heat stress affects the cytoskeleton and the delivery of sucrose synthase in tobacco pollen tubes

Planta
Luigi ParrottaGiampiero Cai

Abstract

Heat stress changes isoform content and distribution of cytoskeletal subunits in pollen tubes affecting accumulation of secretory vesicles and distribution of sucrose synthase, an enzyme involved in cell wall synthesis. Plants are sessile organisms and are therefore exposed to damages caused by the predictable increase in temperature. We have analyzed the effects of temperatures on the development of pollen tubes by focusing on the cytoskeleton and related processes, such as vesicular transport and cell wall synthesis. First, we show that heat stress affects pollen germination and, to a lesser extent, pollen tube growth. Both, microtubules and actin filaments, are damaged by heat treatment and changes of actin and tubulin isoforms were observed in both cases. Damages to actin filaments mainly concern the actin array present in the subapex, a region critical for determining organelle and vesicle content in the pollen tube apex. In support of this, green fluorescent protein-labeled vesicles are arranged differently between heat-stressed and control samples. In addition, newly secreted cell wall material (labeled by propidium iodide) shows an altered distribution. Damage induced by heat stress also extends to proteins that bind ac...Continue Reading

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Citations

Apr 14, 2016·Plant Reproduction·Florian Müller, Ivo Rieu
Nov 11, 2016·PloS One·Iris HeidmannMarco Di Berardino
Feb 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhen-Yu QiJie Zhou
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Aug 1, 2020·Protoplasma·Aslıhan Çetinbaş-Genç, Filiz Vardar
Apr 17, 2018·Annals of Botany·Luigi ParrottaGiampiero Cai
Oct 14, 2020·Plant, Cell & Environment·Farhad Masoomi-AladizgehBrian J Atwell
Jan 21, 2021·International Journal of Molecular Sciences·Lavinia MareriGiampiero Cai
Apr 6, 2021·The New Phytologist·Palak ChaturvediDavid Honys
Aug 28, 2021·International Journal of Molecular Sciences·Lavinia MareriGiampiero Cai

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