Heat treatment could affect the biochemical properties of caldesmon.

The Journal of Biological Chemistry
S ZhuangC A Wang

Abstract

Smooth muscle caldesmon (CaD) exhibits apparent heat stability. A widely used purification procedure of CaD involves extensive heat treatment (Bretscher, A. (1984) J. Biol. Chem. 259, 12873-12880). CaD thus purified co-sediments with actin, inhibits actomyosin ATPase activity, and interacts with Ca2+/calmodulin, similarly to the unheated protein. On the other hand, heat-treated CaD binds to actin filaments in a tether-like fashion, whereas lengthwise binding dominates in vivo (Mabuchi, K., Lin, J. J.-C., and Wang, C.-L. A. (1993) J. Muscle Res. Cell Motil. 14, 54-64), suggesting that differences do exist between heat-purified CaD and the native protein. We have isolated, without heat treatment, full-length recombinant chicken gizzard CaD overexpressed in insect cells (High-FiveTM) using a baculovirus expression system. We found that such unheated CaD interacts with calmodulin 10 times stronger than does the heated CaD; its inhibitory action on actomyosin ATPase is reversed by a much lesser amount of calmodulin. Moreover, electron microscopic examination indicated that actin binding at the N-terminal region is more frequent in the unheated CaD, resulting in more lengthwise binding. These findings point to the fact that CaD is no...Continue Reading

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Citations

Aug 12, 2014·Biochimica Et Biophysica Acta·Horia Nicolae RomanAnne-Marie Lauzon
Jan 15, 1999·Acta Physiologica Scandinavica·J M ChalovichY D Chen
Apr 8, 2009·International Review of Cell and Molecular Biology·Jim Jung-Ching LinJian-Ping Jin
Nov 6, 2009·The Journal of Biological Chemistry·Renjian HuangChih-Lueh Albert Wang
Feb 7, 2001·The Journal of Biological Chemistry·Y LiC A Wang
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