Heat treatment dependent cytotoxicity of silicalite-1 films deposited on Ti-6Al-4V alloy evaluated by bone-derived cells.

Scientific Reports
Ivana NemcakovaLucie Bacakova

Abstract

A silicalite-1 film (SF) deposited on Ti-6Al-4V alloy was investigated in this study as a promising coating for metallic implants. Two forms of SFs were prepared: as-synthesized SFs (SF-RT), and SFs heated up to 500 °C (SF-500) to remove the excess of template species from the SF surface. The SFs were characterized in detail by X-ray photoelectron spectroscopy (XPS), by Fourier transform infrared spectroscopy (FTIR), by scanning electron microscopy (SEM) and water contact angle measurements (WCA). Two types of bone-derived cells (hFOB 1.19 non-tumor fetal osteoblast cell line and U-2 OS osteosarcoma cell line) were used for a biocompatibility assessment. The initial adhesion of hFOB 1.19 cells, evaluated by cell numbers and cell spreading area, was better supported by SF-500 than by SF-RT. While no increase in cell membrane damage, in ROS generation and in TNF-alpha secretion of bone-derived cells grown on both SFs was found, gamma H2AX staining revealed an elevated DNA damage response of U-2 OS cells grown on heat-treated samples (SF-500). This study also discusses differences between osteosarcoma cell lines and non-tumor osteoblastic cells, stressing the importance of choosing the right cell type model.

References

Feb 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S A HarrisT C Spelsberg
May 1, 1996·Reproductive Toxicology·J L Domingo
Apr 13, 2000·The Journal of Bone and Joint Surgery. American Volume·R M UrbanM Peoc'h
Apr 14, 2000·Frontiers in Bioscience : a Journal and Virtual Library·K Somasundaram
Apr 27, 2000·Nature Cell Biology·J W Harbour, D C Dean
Feb 24, 2001·The Journal of Bone and Joint Surgery. American Volume·C VermesT T Glant
Mar 7, 2001·Cellular and Molecular Life Sciences : CMLS·E Hulleman, J Boonstra
Jul 10, 2003·Cancer Genetics and Cytogenetics·K Al-RomaihJ A Squire
Nov 19, 2004·The Journal of Biological Chemistry·Gerald W HsuLorena S Beese
Nov 24, 2004·Colloids and Surfaces. B, Biointerfaces·Kalpana S Katti
Jul 13, 2006·Expert Review of Anticancer Therapy·Alexander J Chou, Richard Gorlick
Aug 24, 2006·Angewandte Chemie·Zijian LiYushan Yan
Apr 4, 2007·The Journal of Bone and Joint Surgery. American Volume·Steven KurtzMichael Halpern
Apr 29, 2008·Biomaterials·David F Williams
Aug 5, 2008·Cell Biology International·Dingsheng LinWeishan Chen
Jun 10, 2009·Journal of Nanoscience and Nanotechnology·Lubica GrausovaVera Lisa
Jun 18, 2010·Neurotoxicology·Stephen C Bondy
Oct 5, 2010·Biological Trace Element Research·Xinwei LiZhigang Zhang
Feb 18, 2011·Journal of Bone and Mineral Metabolism·María del Pilar MartínezMaría Inés Conti
Jun 4, 2011·Genetics and Molecular Biology·Cristiano C GomesFabio V Santos
Feb 14, 2012·Acta Biomaterialia·Arne BiesiekierskiCuie Wen
Apr 30, 2013·International Journal of Molecular Sciences·Ivana KopovaJiri Vacik
May 15, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Ralf J KohalFrank Butz
Jul 17, 2013·Clinical Cases in Mineral and Bone Metabolism : the Official Journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases·Valerio SansoneMarco Melato
Jul 28, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Yun-Ping BaoHua-Song Luo
Aug 29, 2013·Journal of Biomedical Materials Research. Part a·E M CzekanskaJ S Hayes
Jan 13, 2015·Journal of Biomechanics·D R Sumner
Mar 12, 2015·Biochemical and Biophysical Research Communications·Yong LiZheng Guo
Jun 30, 2016·Electromagnetic Biology and Medicine·Anna-Maria BiqueMervi Paulasto-Kröckel

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
Scanning
X-ray
ELISA
flow cytometry

Software Mentioned

SigmaStat
SigmaPlot
Atlas

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.