Heating after intense repeated contractions inhibits glycogen accumulation in mouse EDL muscle: role of phosphorylase in postexercise glycogen metabolism

American Journal of Physiology. Cell Physiology
Sarah J BlackwoodAbram Katz

Abstract

The effects of heating on glycogen synthesis (incorporation of [14C]glucose into glycogen) and accumulation after intense repeated contractions were investigated. Isolated mouse extensor digitorum longus muscle (type II) was stimulated electrically to perform intense tetanic contractions at 25°C. After 120 min recovery at 25°C, glycogen accumulated to almost 80% of basal, whereas after recovery at 35°C, glycogen remained low (~25% of basal). Glycogen synthesis averaged 0.97 ± 0.07 µmol·30 min-1·g wet wt-1 during recovery at 25°C and 1.48 ± 0.08 during recovery at 35°C ( P < 0.001). There were no differences in phosphorylase and glycogen synthase total activities nor in phosphorylase fractional activity, whereas glycogen synthase fractional activity was increased by ~50% after recovery at 35°C vs. 25°C. Inorganic phosphate (Pi, substrate for phosphorylase) was markedly increased (~300% of basal) following contraction but returned to control levels after 120 min recovery at 25°C. In contrast, Pi remained elevated after recovery at 35°C (>2-fold higher than recovery at 25°C). Estimates of glycogen breakdown indicated that phosphorylase activity (either via inhibition at 25°C or activation at 35°C) was responsible for ~60% of glyco...Continue Reading

References

Oct 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·K SahlinE Hultman
Nov 1, 1977·The Journal of Physiology·R H EdwardsP A Merton
Sep 1, 1976·European Journal of Biochemistry·H G NimmoP Cohen
Dec 28, 1976·Pflügers Archiv : European journal of physiology·R C HarrisK Sahlin
Mar 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L N Johnson
Feb 16, 1987·European Journal of Biochemistry·R A ChallissE A Newsholme
Jun 1, 1987·The American Journal of Physiology·J L IvyO H Lowry
Nov 1, 1985·Biophysical Journal·R Cooke, E Pate
Jun 1, 1966·The American Journal of Physiology·F A Fuhrman, G J Fuhrman
May 12, 1970·Biochemistry·D L DeVincenzi, J L Hedrick
Oct 1, 1967·Acta Physiologica Scandinavica·L HermansenB Saltin
Oct 1, 1967·Acta Physiologica Scandinavica·J BergströmB Saltin
May 9, 2002·American Journal of Physiology. Cell Physiology·Ingrid HelanderAbram Katz
Apr 16, 2004·Pflügers Archiv : European journal of physiology·Marie E SandströmAbram Katz
Nov 12, 2005·American Journal of Physiology. Cell Physiology·E P DeboldR H Fitts
Dec 3, 2005·Journal of Muscle Research and Cell Motility·C J Barclay
Sep 26, 2006·Biochemical and Biophysical Research Communications·Anna WredenbergNils-Göran Larsson
Dec 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·M LiuK Vandenborne
Dec 23, 2006·The Journal of Physiology·Marie E SandströmAbram Katz
Jul 7, 2007·American Journal of Physiology. Cell Physiology·Shi-Jin ZhangAbram Katz
Jan 16, 2008·Physiological Reviews·D G AllenH Westerblad
Nov 3, 2010·The Journal of Physiology·Graham D Lamb, Håkan Westerblad
Mar 14, 2012·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Dustin SlivkaBrent Ruby
Dec 19, 2012·Frontiers in Physiology·David G Allen, Sofie Trajanovska
Aug 6, 2013·Pflügers Archiv : European journal of physiology·Abram KatzHåkan Westerblad
Apr 30, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Abram Katz, Håkan Westerblad
Jul 19, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sigitas KamandulisHåkan Westerblad

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
force measurements
PCA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.