Heavy and light chain sequences of four monoclonal antibodies that bind galactosylgloboside (GalGb4)

European Journal of Immunology
J G SnyderD M Marcus

Abstract

We recently described IgM monoclonal antibodies directed against the glycospingolipid galactosylgloboside (GalGb4; Marcus, M. D. et al., Arch. Biochem. Biophys, 1988.262: 620). We now present the nucleotide and deduced amino acid sequences of the heavy and light chains of these antibodies. The antibodies were generated in a single fusion, their heavy as well as their light chains are almost identical, and they appear to be clonally related. The light chains were encoded by J kappa 5 and a V kappa gene belonging to the Ox1 family, but they are only 93% homologous to the most closely related germ-line gene, and they are probably encoded by a germ-line gene that has not yet been identified. The heavy chains were all encoded by VH441 and JH2, and have identical N segments. The VH441 germ-line gene encodes a potential glycosylation site at Asn58 in the complementarity-determining region 2. This site, which has been retained in all VH441-encoded monoclonal antibodies sequenced previously, was mutated out by a single base change in all four anti-GalGb4 antibodies.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1988·European Journal of Immunology·M KaartinenO Mäkelä
May 1, 1988·Archives of Biochemistry and Biophysics·D M MarcusA Suzuki
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J GeliebterS G Nathenson
Nov 1, 1983·The Journal of Experimental Medicine·S RudikoffM Potter

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