Heavy Chronic Intermittent Ethanol Exposure Alters Small Noncoding RNAs in Mouse Sperm and Epididymosomes

Frontiers in Genetics
Gregory R RompalaGregg E Homanics

Abstract

While the risks of maternal alcohol abuse during pregnancy are well-established, several preclinical studies suggest that chronic preconception alcohol consumption by either parent may also have significance consequences for offspring health and development. Notably, since isogenic male mice used in these studies are not involved in gestation or rearing of offspring, the cross-generational effects of paternal alcohol exposure suggest a germline-based epigenetic mechanism. Many recent studies have demonstrated that the effects of paternal environmental exposures such as stress or malnutrition can be transmitted to the next generation via alterations to small noncoding RNAs in sperm. Therefore, we used high throughput sequencing to examine the effect of preconception ethanol on small noncoding RNAs in sperm. We found that chronic intermittent ethanol exposure altered several small noncoding RNAs from three of the major small RNA classes in sperm, tRNA-derived small RNA (tDR), mitochondrial small RNA, and microRNA. Six of the ethanol-responsive small noncoding RNAs were evaluated with RT-qPCR on a separate cohort of mice and five of the six were confirmed to be altered by chronic ethanol exposure, supporting the validity of the se...Continue Reading

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Dec 26, 2018·Journal of Neuroendocrinology·Hirotaka Hamada, Stephen G Matthews
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Datasets Mentioned

BETA
PRJNA414349

Methods Mentioned

BETA
acetylation
Transmission electron microscopy
protein exchange
light microscopy
electrophoresis
PCR

Software Mentioned

TargetScan Mouse Custom
DESeq2
MassLynx
DAVID Bioinformatics Resources
Originlab
RNAhybrid
Bowtie2
Python script
Cutadapt
tDRmapper

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