Heavy metal accumulation by recombinant mammalian metallothionein within Escherichia coli protects against elevated metal exposure

Chemosphere
Sandrine Sauge-MerleMichael Dubow

Abstract

Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich, small proteins known to provide protection against toxic heavy metals such as cadmium. In an attempt to increase the ability of bacterial cells to accumulate heavy metals, sheep MTII was produced in fusion with the maltose binding protein (MBP) and localized to the cytoplasmic or periplasmic compartments of Escherichia coli. For all metals tested, higher levels of bioaccumulation were measured with strains over-expressing MBP-MT in comparison with control strains. A marked bioaccumulation of Cd, As, Hg and Zn was observed in the strain over-expressing MBP-MT in the cytoplasm, whereas Cu was accumulated to higher levels when MBP-MT was over-expressed in the periplasm. Metal export systems may also play a role in this bioaccumulation. To illustrate this, we over-expressed MBP-MT in the cytoplasm of two mutant strains of E. coli affected in metal export. The first, deficient in the transporter ZntA described to export numerous divalent metal ions, showed increasing quantities of Zn, Cd, Hg and Pb being bioaccumulated. The second, strain LF20012, deficient in As export, showed that As was bioaccumulated in the form of arsenite. Furthermore, high quantities of accum...Continue Reading

References

Feb 1, 1995·Journal of Industrial Microbiology·D H Nies, S Silver
Jul 1, 1996·Canadian Journal of Microbiology·J Cai, M S DuBow
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
May 16, 1998·The Journal of Biological Chemistry·J M BettonM Laurent
Aug 1, 1996·Nature Biotechnology·C SousaV de Lorenzo
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D Palmiter
Jul 28, 1999·Applied Microbiology and Biotechnology·D H Nies
Sep 26, 2001·Current Genetics·M J Tamás, R Wysocki
Aug 8, 2002·FEMS Microbiology Reviews·Rita MukhopadhyaySimon Silver
Jan 7, 2003·Applied and Environmental Microbiology·Sandrine Sauge-MerleGilles Peltier
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
Mar 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Richard D Palmiter
May 3, 2005·Annual Review of Plant Biology·Elizabeth Pilon-Smits
Oct 20, 2005·Journal of Bioscience and Bioengineering·Se-Kwon KimEun-Ki Kim
Feb 8, 2006·Applied and Environmental Microbiology·Cindy H WuWilfred Chen
Oct 24, 2006·Journal of Inorganic Biochemistry·Kelly E Rigby Duncan, Martin J Stillman
Sep 22, 2007·Science·Dietrich H Nies
Jan 4, 2008·Journal of Inorganic Biochemistry·Claudia A Blindauer
Jun 10, 2008·Journal of Bacteriology·Kerstin HelbigDietrich H Nies
Dec 17, 2008·Nature Reviews. Microbiology·Kevin J Waldron, Nigel J Robinson

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Citations

Jun 26, 2013·Journal of Inorganic Biochemistry·Attila JancsóRóbert Berkecz
Jan 26, 2016·Inorganic Chemistry·Celin Acharya, Claudia A Blindauer
Mar 5, 2016·Bioscience, Biotechnology, and Biochemistry·Chengyuan WangBeijuan Hu
Oct 28, 2017·Physical Chemistry Chemical Physics : PCCP·Romain M PrésentJérôme F L Duval
May 21, 2020·Frontiers in Microbiology·Sergio BalzanoChristophe Brunet
Nov 14, 2018·Frontiers in Bioengineering and Biotechnology·Patrick DiepAlexander F Yakunin
Apr 28, 2017·Environmental Geochemistry and Health·Zahra Derakhshan NejadKi-Hyun Kim
Feb 3, 2021·Environmental Science and Pollution Research International·Anindita MitraDharmendra K Gupta
Nov 16, 2021·Bioengineered·Sharrel RebelloAshok Pandey

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