Abstract
Prokaryotic enzymes formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease VIII (Nei) and their eukaryotic homologs NEIL1, NEIL2, and NEIL3 define the Fpg family of DNA glycosylases, which initiate the process of repair of oxidized DNA bases. The repair of oxidative DNA lesions is known to be impaired in vivo in the presence of ions of some heavy metals. We have studied the effect of salts of several alkaline earth and transition metals on the activity of Fpg-family DNA glycosylases in the reaction of excision of 5,6-dihydrouracil, a typical DNA oxidation product. The reaction catalyzed by NEIL1 was characterized by values K(m) = 150 nM and k(cat) = 1.2 min(-1), which were in the range of these constants for excision of other damaged bases by this enzyme. NEIL1 was inhibited by Al3+, Ni2+, Co2+, Cd2+, Cu2+, Zn2+, and Fe2+ in Tris-HCl buffer and by Cd2+, Zn2+, Cu2+, and Fe2+ in potassium phosphate buffer. Fpg and Nei, the prokaryotic homologs of NEIL1, were inhibited by the same metal ions as NEIL1. The values of I(50) for NEIL1 inhibition were 7 microM for Cd2+, 16 microM for Zn2+, and 400 microM for Cu2+. The inhibition of NEIL1 by Cd2+, Zn2+, and Cu2+ was at least partly due to the formation of metal-DNA complexes. In the...Continue Reading
References
Jul 1, 1989·Biological Trace Element Research·A Hartwig, D Beyersmann
Jan 21, 1985·Biochimica Et Biophysica Acta·P J Thornalley, M Vasák
Dec 18, 1968·Journal of the American Chemical Society·G L Eichhorn, Y A Shin
Nov 16, 1993·Biochemistry·M DizdarogluS Boiteux
Nov 1, 1993·Biophysical Journal·J DuguidG J Thomas
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K T TamaiD J Thiele
Apr 22, 1993·Nature·T Lindahl
Jun 1, 1997·Journal of Bacteriology·D JiangS S Wallace
Nov 14, 1997·Toxicology and Applied Pharmacology·T HiranoH Kasai
May 20, 1999·Annual Review of Pharmacology and Toxicology·C D KlaassenS Choudhuri
Mar 8, 2000·Nature·S D BrunerG L Verdine
Mar 18, 2000·Toxicology Letters·M AsmussA Hartwig
Jun 1, 2000·Journal of the American Society for Mass Spectrometry·R A RiegerC R Iden
Nov 4, 2000·Carcinogenesis·M AsmussA Hartwig
Feb 16, 2002·The EMBO Journal·Dmitry O ZharkovGil Shoham
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tapas K HazraSankar Mitra
Mar 26, 2002·The Journal of Biological Chemistry·Rotem GilboaGil Shoham
Apr 10, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Montserrat Soler-LópezJuan A Subirana
Jun 12, 2002·Biochemistry·Alexander A IshchenkoGeorgy A Nevinsky
Jan 2, 2003·DNA Repair·Dmitry O Zharkov, Thomas A Rosenquist
Apr 26, 2003·DNA Repair·Thomas A RosenquistArthur P Grollman
Jun 5, 2003·Mechanisms of Ageing and Development·Irina G PopovichAnatoli I Yashin
Aug 2, 2003·DNA Repair·Dmitry O ZharkovArthur P Grollman
Sep 25, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Shaunivan L LabiukJeremy S Lee
Nov 11, 2003·The Journal of Biological Chemistry·Elena I ZaikaDmitry O Zharkov
Jun 25, 2004·Nucleic Acids Research·Vladimir A IvanisenkoNickolay A Kolchanov
Jul 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sylvie DoubliéSusan S Wallace
Jul 22, 2004·European Journal of Biochemistry·Holger BlessingAndrea Hartwig
Dec 15, 2004·Biochemistry·Pawel JarugaMiral Dizdaroglu
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Vladimir VartanianR Stephen Lloyd
Feb 17, 2006·DNA Repair·Errol C Friedberg
Sep 28, 2006·Biochemistry·Konstantin Y KropachevArthur P Grollman
Dec 13, 2006·Free Radical Biology & Medicine·Li-Fang ZhengZhong-Li Liu
Jan 9, 2007·DNA Repair·Kenichi HitomiJohn A Tainer
Mar 6, 2007·Biochemistry·Sandeep KumarMing-Daw Tsai
Nov 1, 2007·Chemical Research in Toxicology·Konstantin Salnikow, Anatoly Zhitkovich
Feb 5, 2008·Molekuliarnaia biologiia·D O Zharkov
May 23, 2008·Archives of Toxicology·Detmar Beyersmann, Andrea Hartwig
Jun 19, 2008·The FEBS Journal·Viktoriya S SidorenkoDmitry O Zharkov
Nov 8, 2008·Molekuliarnaia biologiia·V S Sidorenko, D O Zharkov
Citations
Mar 19, 2014·Analytica Chimica Acta·Chen LiHaizhen Zhu
Jul 14, 2010·The Journal of Biological Chemistry·Muralidhar L HegdeSankar Mitra
Mar 27, 2012·Mutagenesis·J KainL Möller
May 17, 2011·Biochemistry. Biokhimii︠a︡·I R Grin, D O Zharkov
Nov 19, 2013·Biochemistry. Biokhimii︠a︡·L V GeningV Z Tarantul
Oct 5, 2010·The Science of the Total Environment·Hongmei WangShen Ke
Jul 13, 2016·Analytical Chemistry·Li-Juan WangChun-Yang Zhang
Sep 25, 2016·Mechanisms of Ageing and Development·Haibo WangMuralidhar L Hegde
Jun 18, 2017·Analytical Chemistry·Yan ZhangChun-Yang Zhang
May 19, 2018·Environmental Science. Processes & Impacts·Wen-Xiong WangNanyan Weng
Aug 29, 2018·Journal of Alzheimer's Disease : JAD·Sakae YumotoAkira Ishikawa
Jan 22, 2019·The Analyst·Wenya DangWei Wang
Jan 17, 2020·Metallomics : Integrated Biometal Science·Chao XingXiaofang Li
Jun 28, 2018·Molecular Neurobiology·Kowsar BavarsadAmirhossein Sahebkar
Aug 19, 2017·BMC Complementary and Alternative Medicine·Xin-Shi WangCheng-Long Xie
Apr 10, 2018·Chemical Science·Juan HuChun-Yang Zhang
May 1, 2021·Analytical Chemistry·Juan HuChun-Yang Zhang
Jan 25, 2018·ACS Sensors·Jizhen ShangHua Wang
Nov 22, 2018·ACS Sensors·Li-Juan WangChun-Yang Zhang
Jan 21, 2022·Analytical Chemistry·Qian ZhangChun-Yang Zhang