Heavy metals generate reactive oxygen species in terrestrial and aquatic ciliated protozoa

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Daniel RicoJuan-Carlos Gutiérrez

Abstract

Reactive oxygen species (ROS) induction by exposure to heavy metals (Cd, Cu or Zn) in diverse free-living ciliated protozoa (Tetrahymena sp. and three strains of Colpoda steinii, isolated from freshwater and soils with different level of metal pollution) has been evaluated. Using specific fluorophores, such as 2',7'-dichlorofluorescein diacetate, hydroethidine and dihydrorhodamine 123, and a fluorescence microscope with the program MetaMorph Imaging System 4.0, we have analyzed both the average fluorescence emission and the heterogeneous distribution of fluorescence in control and treated cells. This is the first time that these fluorophores are used to detect ROS production in ciliated protozoa. All metals generate ROS, mainly superoxide and peroxides, showing a remarkable inter- and intra-specific variations. Likewise, resistance against each metal was also very diverse. Cu and specially Cd, the most toxic heavy metal for these ciliates, are the best oxidative stress inducers. However, a correlation between fluorescence emission intensity and cellular metal sensitivity for each strain cannot be established. Results are discussed and compared with similar findings previously published in other unicellular and pluricellular org...Continue Reading

References

Sep 23, 1996·Mutation Research·R J Brennan, R H Schiestl
Jul 1, 1997·Ecotoxicology and Environmental Safety·M P SauvantJ Guillot
Nov 21, 1998·Free Radical Biology & Medicine·L BenovI Fridovich
Mar 10, 1999·Chemosphere·M P SauvantE Piccinni
Apr 28, 1999·Free Radical Biology & Medicine·D J TateD A Newsome
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph
Jun 29, 2001·Environmental Pollution·A HeikensA J Hendriks
Oct 11, 2001·Biological Chemistry·H HaaseD Beyersmann
Apr 27, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Masumi Watanabe, Tetsuya Suzuki
May 9, 2002·Journal of Experimental Botany·Andres Schützendübel, Andrea Polle
Dec 4, 2003·Mutation Research·Marisa D Pulido, Alan R Parrish
Aug 25, 2004·Environmental Pollution·J AguilarM Simón
Mar 19, 2005·Cell Biology International·Ana Martín-GonzálezJuan Carlos Gutiérrez
Apr 7, 2005·Journal of Basic Microbiology·Stefan FujsMartin Batic
May 11, 2005·Toxicology and Applied Pharmacology·Gerhard KrumschnabelBettina Hofer
May 17, 2005·Current Medicinal Chemistry·M ValkoM T D Cronin
Sep 1, 2005·Research in Microbiology·Ana Martín-GonzálezJuan Carlos Gutiérrez
Oct 4, 2005·Environmental Pollution·Paolo Madoni, Maria Giuseppa Romeo
Nov 22, 2005·Journal of Biochemical and Biophysical Methods·Ana GomesJosé L F C Lima
May 3, 2006·Environment International·Silvia DíazJuan Carlos Gutiérrez
Aug 25, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Alessia FormigariAlessandro Santon
Dec 8, 2007·Aquatic Toxicology·Stephanie K BoppKatja Knauer

❮ Previous
Next ❯

Citations

Jun 9, 2009·Journal of Bacteriology·William LainhartGerald B Koudelka
Jul 19, 2011·Ecotoxicology and Environmental Safety·José Sebastián Dávila CostaCarlos Mauricio Abate
Oct 5, 2010·Journal of Hazardous Materials·Tserennyam BatjargalJung-Seok Yang
Sep 1, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mazyar Yazdani
Nov 30, 2014·Bulletin of Environmental Contamination and Toxicology·Mazyar YazdaniKetil Hylland
Jul 25, 2014·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bo-Mi KimJae-Seong Lee
Nov 18, 2014·TheScientificWorldJournal·Mohd Khalizan SabullahSiti Aqlima Ahmad
Oct 11, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bo-Mi KimJae-Seong Lee
Jul 2, 2016·European Journal of Protistology·Vera SlaveykovaJuan Carlos Gutiérrez
May 23, 2009·Toxicology·Richard R Chapleau, Martin Sagermann
Mar 10, 2017·International Journal of Environmental Research and Public Health·Guangyi SunXinbin Feng
Feb 15, 2018·Environmental Toxicology and Chemistry·Carly J Nowicki, Donna R Kashian
Nov 9, 2018·Bulletin of Environmental Contamination and Toxicology·S VincentJ Prabha
May 23, 2020·Environmental Science and Pollution Research International·Samuel David Raj ThatipakaSuman Polaki
Mar 7, 2021·International Journal of Environmental Research and Public Health·Granit KastratiMihone Kerolli Mustafa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis