PMID: 9431812Feb 12, 1998Paper

Hedgehog signaling regulates transcription through Gli/Ci binding sites in the wingless enhancer

Mechanisms of Development
T Von Ohlen, J E Hooper

Abstract

The segment polarity gene cubitus interruptus (ci) encodes a transcriptional effector of Hedgehog (Hh) signaling in Drosophila. The Ci gene product is a zinc finger protein belonging to the Gli family of sequence-specific DNA binding proteins. After gastrulation, segmental expression of the segment polarity gene wingless (wg) is maintained by Hh signaling in a pathway requiring Ci activity. In the absence of Hh or Ci activity, wg expression is initiated normally and then fades in the ectoderm after stage 10. We have previously identified a wingless enhancer region whose Ci binding sites mediate Ci-dependent transcriptional activation in transiently transfected cells. Here we demonstrate that Hh and Patched (Ptc) act through those Ci binding sites to modulate the level of Ci-dependent transcriptional activation in S2 cells. We demonstrate that this same wg enhancer region is Hh responsive in vivo and that its Ci binding sites are necessary for its activity. This provides strong evidence that Hh affects wg transcription through post-translational activation of Ci.

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Citations

Mar 29, 2001·The Journal of Pathology·G Saldanha
Apr 24, 2004·Biochemical Pharmacology·Stacey K OgdenDavid J Robbins
Sep 8, 1998·Current Opinion in Genetics & Development·R L Johnson, M P Scott
Mar 11, 1999·Current Opinion in Genetics & Development·P Polakis
Jan 5, 2012·Molecular Pharmaceutics·Ryan R HurtadoThomas J Meade
Jul 3, 1998·The EMBO Journal·P W Ingham
Apr 25, 2008·Journal of the Royal Society, Interface·Madalena Chaves, Réka Albert
Nov 13, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Andrea I Ramos, Scott Barolo
Oct 18, 2012·Science Signaling·David J RobbinsNatalia A Riobo
Sep 15, 2000·Biological Chemistry·S KaeslerU Rüther
Sep 1, 2014·Genome Biology and Evolution·Majid KazemianSaurabh Sinha
Oct 4, 2015·Trends in Pharmacological Sciences·Angela M ArensdorfStacey K Ogden
Nov 2, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P S PereiraF Casares
Sep 27, 2012·Molecular Systems Biology·Michael A WhiteBarak A Cohen
Jan 11, 2002·Developmental Biology·Corinne AngelatsStephen Kerridge
Jan 12, 1999·Neuron·L V Goodrich, M P Scott
Jul 26, 2002·The Journal of Biological Chemistry·Junhao MaoDianqing Wu

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