Hedyotis diffusa Willd. extract suppresses proliferation and induces apoptosis via IL-6-inducible STAT3 pathway inactivation in human colorectal cancer cells

Oncology Letters
Jiumao LinJun Peng

Abstract

Recent studies have indicated that the inflammatory microenvironment plays a significant role in colorectal cancer (CRC). The interleukin-6/signal transducer and activator of transcription 3 (IL-6/STAT3) signaling pathway mediates the proliferative and anti-apoptotic activities required for oncogenesis under inflammatory conditions; thus, suppressing tumor growth by targeting the IL-6/STAT3 pathway is a promising therapeutic strategy for CRC. Our previous study reported that the ethanol extract obtained from Hedyotis diffusa Willd. (EEHDW) can induce apoptosis, and inhibit the proliferation of colon cancer cells and tumor angiogenesis by modulating various signaling pathways; however, less is known regarding the activity of EEHDW in a cancer-promoting inflammatory environment. Therefore, the present study investigated whether EEHDW inhibits the growth of the CRC HT-29 cell line via the IL-6/STAT3 signaling pathway. Pretreatment of HT-29 cells with IL-6 led to an increase in cell viability, colony formation and phosphorylated STAT3 (p-STAT3) expression. Treatment of these cells with EEHDW prior to IL-6 stimulation resulted in a significant reduction in the IL-6-induced phosphorylation of STAT3. In addition, EEHDW treatment signi...Continue Reading

References

Sep 12, 1997·Science·J E Darnell
Oct 10, 1998·International Journal of Cancer. Journal International Du Cancer·S ZushiY Matsuzawa
May 30, 2003·The Biochemical Journal·Peter C HeinrichFred Schaper
Feb 3, 2009·Cancer Cell·Jacqueline Bromberg, Timothy C Wang
Sep 3, 2009·Annals of the New York Academy of Sciences·Bharat B AggarwalBokyung Sung
May 21, 2010·Journal of Experimental & Clinical Cancer Research : CR·Chen HuangZhengjun Qiu
Jun 29, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E Van CutsemUNKNOWN ESMO Guidelines Working Group
Jan 24, 2012·Journal of Ethnopharmacology·Shengpeng WangYitao Wang
Jul 4, 2012·International Journal of Molecular Sciences·Qiaoyan CaiJun Peng
Nov 10, 2012·Experimental and Therapeutic Medicine·Minghe LinDezeng Zhu
Apr 23, 2013·Journal of Asian Natural Products Research·Yu Niu, Qiu-Xia Meng
Apr 30, 2013·Journal of Ethnopharmacology·Qiu-Xia MengJane R Hanrahan
Jul 3, 2013·Neoplasia : an International Journal for Oncology Research·Qing ChangJacqueline Bromberg
Jan 17, 2014·International Journal of Oncology·Shu-Wei Wang, Yue-Ming Sun
Mar 19, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Mar 22, 2014·Molecular Cancer Therapeutics·Yun DaiLiang Qiao
Jun 6, 2014·Journal of Immunology Research·Glauben LandskronMarcela A Hermoso
Jul 8, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Ploypailin AneknanLaddawan Senggunprai

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Citations

Dec 19, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Bin LiCheng Guo
Jun 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui ChenMenghua Liu
Mar 23, 2017·World Journal of Gastroenterology : WJG·Jie WangJian-Xin Jiang
Aug 25, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Fan-Yan ZengZeng-Qiong Huang
Aug 29, 2017·Magnetic Resonance in Chemistry : MRC·Zhiyong WangJianxin Chen
May 8, 2020·Oxidative Medicine and Cellular Longevity·Paola AielloWesam Kooti
Sep 28, 2017·Case Reports in Oncological Medicine·Pei-Yuu Yang, Chen-Jei Tai

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Methods Mentioned

BETA
nuclear translocation
protein assay
ELISA
fluorescence-activated cell sorting
FACS
fluorescence microscopy
electrophoresis

Software Mentioned

ModFit LT
SPSS

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