Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules.

The Journal of Biological Chemistry
Byeong-Gu HanByung Il Lee

Abstract

Apoptosis inhibitor 5 (API5) is an anti-apoptotic protein that is up-regulated in various cancer cells. Here, we present the crystal structure of human API5. API5 exhibits an elongated all α-helical structure. The N-terminal half of API5 is similar to the HEAT repeat and the C-terminal half is similar to the ARM (Armadillo-like) repeat. HEAT and ARM repeats have been implicated in protein-protein interactions, suggesting that the cellular roles of API5 may be to mediate protein-protein interactions. Various components of multiprotein complexes have been identified as API5-interacting protein partners, suggesting that API5 may act as a scaffold for multiprotein complexes. API5 exists as a monomer, and the functionally important heptad leucine repeat does not exhibit the predicted a dimeric leucine zipper. Additionally, Lys-251, which can be acetylated in cells, plays important roles in the inhibition of apoptosis under serum deprivation conditions. The acetylation of this lysine also affects the stability of API5 in cells.

References

Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Oct 18, 1996·Cell·E S AlnemriJ Yuan
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Jun 11, 1999·Current Opinion in Structural Biology·M R Groves, D Barford
Apr 26, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·J W KimS E Namkoong
Oct 26, 2000·Nature·P MeierG Evan
Sep 15, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·H SasakiY Fujii
Mar 26, 2002·FEBS Letters·Lei Zeng, Ming Ming Zhou
May 11, 2002·Nature Reviews. Cancer·Frederik H Igney, Peter H Krammer
Jun 4, 2002·Nature Reviews. Molecular Cell Biology·Guy S Salvesen, Colin S Duckett
Mar 18, 2003·The Prostate·Nigel CleggPeter S Nelson
Apr 1, 2003·Molecular and Cellular Biology·Christian SchwerkDanny Reinberg
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Sep 24, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Xiang-Jiao Yang
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Feb 5, 2005·Trends in Biochemical Sciences·Michael J PlevinMitsuhiko Ikura
May 14, 2005·CA: a Cancer Journal for Clinicians·Irene M GhobrialAlex A Adjei
Oct 21, 2006·Molecular Cell·James SampietroWenqing Xu
Nov 23, 2006·PLoS Genetics·Erick J MorrisNicholas J Dyson
Dec 29, 2006·The Journal of Biological Chemistry·Woo-Suk JungSangkee Rhee
Jan 16, 2007·Molecular Cell·Bradley T ScrogginsLen Neckers
Sep 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daqi TuAxel T Brunger
Nov 21, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ning-Fang MaXin-Yuan Guan
Jan 1, 2008·Current Opinion in Structural Biology·Hervé Le Hir, Gregers Rom Andersen
Apr 29, 2008·Molecular Cell·Srinivasa M Srinivasula, Jonathan D Ashwell
Aug 30, 2008·Molecular Cell·Xiang-Jiao Yang, Edward Seto
Apr 14, 2009·Trends in Molecular Medicine·Shi Yu YangMarc C Winslet
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström

❮ Previous
Next ❯

Citations

Oct 16, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Li-Qiang YanZheng-Jian Qi
Mar 25, 2017·EMBO Reports·Gergely ImreKrishnaraj Rajalingam
May 1, 2020·Journal of Virology·Anastassia MikhailovaAsier Sáez-Cirión
Sep 25, 2014·BMB Reports·Kwon-Ho SongTae Woo Kim
Sep 9, 2017·Experimental & Molecular Medicine·Han Sol JangTae Woo Kim
May 10, 2020·Nucleic Acids Research·Seoung Min BongByung Il Lee
Jul 14, 2016·Cancer Research·Léonard Jagot-LacoussiereJean-Luc Poyet
Dec 16, 2020·Protein and Peptide Letters·Meishan ChenQiuxiang Pang
Aug 14, 2021·Scientific Reports·Virender Kumar Sharma, Mayurika Lahiri

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.