Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin

Proceedings of the National Academy of Sciences of the United States of America
Hiroaki MizunoNaoki Watanabe

Abstract

The complex interplay between actin regulatory proteins facilitates the formation of diverse cellular actin structures. Formin homology proteins (formins) play an essential role in the formation of actin stress fibers and yeast actin cables, to which the major actin depolymerizing factor cofilin barely associates. In vitro, F-actin decorated with cofilin exhibits a marked increase in the filament twist. On the other hand, a mammalian formin mDia1 rotates along the long-pitch actin helix during processive actin elongation (helical rotation). Helical rotation may impose torsional force on F-actin in the opposite direction of the cofilin-induced twisting. Here, we show that helical rotation of mDia1 converts F-actin resistant to cofilin both in vivo and in vitro. F-actin assembled by mDia1 without rotational freedom became more resistant to the severing and binding activities of cofilin than freely rotatable F-actin. Electron micrographic analysis revealed untwisting of the long-pitch helix of F-actin elongating from mDia1 on tethering of both mDia1 and the pointed end side of the filament. In cells, single molecules of mDia1ΔC63, an activated mutant containing N-terminal regulatory domains, showed tethering to cell structures mor...Continue Reading

References

Dec 1, 1991·The Journal of Cell Biology·S K MaciverT D Pollard
Apr 1, 1994·The Journal of Cell Biology·J MulhollandD Botstein
Jul 1, 1993·Biophysical Journal·C S PeskinG F Oster
Jan 1, 1993·The Journal of Cell Biology·A L MoonD G Drubin
Aug 17, 1999·Journal of Molecular Biology·A McGough, W Chiu
Nov 13, 1999·Nature Cell Biology·N WatanabeS Narumiya
Dec 28, 1999·Annual Review of Cell and Developmental Biology·J R Bamburg
Feb 9, 2002·Science·Naoki Watanabe, Timothy J Mitchison
Jan 15, 2003·Biochemistry·Martin PringSally H Zigmond
May 31, 2003·Journal of Cell Science·Marie EvangelistaCharles Boone
Mar 27, 2004·Science·Chiharu HigashidaNaoki Watanabe
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·David R Kovar, Thomas D Pollard
Oct 15, 2005·Science·Jian-Qiu Wu, Thomas D Pollard
Oct 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Roland KaunasShu Chien
Feb 24, 2006·The Journal of Biological Chemistry·Beáta BugyiMiklós Nyitrai
Feb 28, 2006·Nature Cell Biology·Nelson A MedeirosPaul Forscher
Jul 11, 2006·Biophysical Journal·Gábor PappMiklós Nyitrai
Oct 5, 2006·Molecular Cell·Ernesto Andrianantoandro, Thomas D Pollard
Mar 22, 2007·Annual Review of Biochemistry·Bruce L Goode, Michael J Eck
May 12, 2007·Current Biology : CB·Alphée MichelotLaurent Blanchoin
Sep 19, 2007·The Journal of Cell Biology·Voytek Okreglak, David G Drubin
Oct 2, 2008·Journal of Cell Science·Chiharu HigashidaNaoki Watanabe
Jan 23, 2009·Nature·Toshiro OdaAkihiro Narita
Feb 27, 2009·Molecular Biology of the Cell·Colleen T SkauDavid R Kovar
Jan 16, 2010·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Naoki Watanabe
Jul 28, 2010·Molecular Biology of the Cell·Sadanori WatanabeShuh Narumiya
Sep 4, 2010·Cytoskeleton·Matthew B SmithDimitrios Vavylonis
Jul 5, 2011·Biophysical Journal·Brannon R McCulloughEnrique M De La Cruz
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vitold E GalkinEdward H Egelman

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Citations

Nov 6, 2018·Biophysics Reviews·Naomi Courtemanche
May 6, 2019·Journal of Muscle Research and Cell Motility·Dietmar J MansteinPeter W Gunning
Jul 18, 2019·Journal of Muscle Research and Cell Motility·Sawako Yamashiro, Naoki Watanabe
Nov 22, 2019·Journal of Muscle Research and Cell Motility·Hugo WiolandAntoine Jegou
May 14, 2020·Biomolecules·Mizuki MatsuzakiAkihiro Narita
May 31, 2018·Molecular Biology of the Cell·Sawako YamashiroNaoki Watanabe
Jan 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Hugo WiolandGuillaume Romet-Lemonne
May 7, 2020·International Journal of Molecular Sciences·Kiyotaka TokurakuTaro Q P Uyeda
Jul 28, 2019·Journal of Muscle Research and Cell Motility·Akihiro Narita
Oct 7, 2020·Annual Review of Cell and Developmental Biology·Rachel S KadzikDavid R Kovar
Nov 27, 2020·Nature Communications·Andrew R HarrisDaniel A Fletcher
Dec 22, 2019·Seminars in Cell & Developmental Biology·Antoine Jegou, Guillaume Romet-Lemonne
Nov 8, 2020·Current Opinion in Cell Biology·Antoine Jégou, Guillaume Romet-Lemonne
Apr 3, 2021·Frontiers in Cell and Developmental Biology·Gaël MénaschéUlrich Blank
May 25, 2021·Cytoskeleton·Caitlin A AndersonJonathan D Winkelman
Dec 5, 2019·Nano Letters·Emiko L SuzukiAntoine Jégou

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Methods Mentioned

BETA
GTPase
Fluorescence
GTPases
Transfection

Software Mentioned

Speckle Tracker J
ImageJ

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