PMID: 9528681Apr 7, 1998Paper

Helicity, membrane incorporation, orientation and thermal stability of the large conductance mechanosensitive ion channel from E. coli

Biochimica Et Biophysica Acta
I T ArkinA T Brünger

Abstract

In this report, we present structural studies on the large conductance mechanosensitive ion channel (MscL) from E. coli in detergent micelles and lipid vesicles. Both transmission Fourier transform infrared spectroscopy and circular dichroism (CD) spectra indicate that the protein is highly helical in detergents as well as liposomes. The secondary structure of the proteins was shown to be highly resistant towards denaturation (25-95 degrees C) based on an ellipticity thermal profile. Amide H+/D+ exchange was shown to be extensive (ca. 66%), implying that two thirds of the protein are water accessible. MscL, reconstituted in oriented lipid bilayers, was shown to possess a net bilayer orientation using dichroic ratios measured by attenuated total-reflection Fourier transform infrared spectroscopy. Here, we present and discuss this initial set of structural data on this new family of ion-channel proteins.

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Citations

Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·X OuC Kung
Feb 26, 2003·The Journal of General Physiology·Andriy AnishkinSergei Sukharev
Nov 5, 1999·Protein Science : a Publication of the Protein Society·A J OakleyM C Wilce
Aug 4, 2015·European Biophysics Journal : EBJ·Gamma ChiMichael J Landsberg
Jun 23, 1999·Structure·A F BatizaC Kung
Sep 3, 2004·Journal of Molecular Biology·William C Wimley, Stephen H White
Oct 9, 1998·The Journal of Biological Chemistry·S Corey, D E Clapham
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Sukharev
Jan 17, 2020·Microbiology and Molecular Biology Reviews : MMBR·Paul Blount, Irene Iscla
Jul 7, 1999·Biochimica Et Biophysica Acta·E GoormaghtighJ M Ruysschaert
May 23, 2006·Archives of Biochemistry and Biophysics·Conceição A S A Minetti, David P Remeta
Jun 30, 2018·The Journal of Physical Chemistry Letters·Esther S Brielle, Isaiah T Arkin

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