Helicity of alpha(404-451) and beta(394-445) tubulin C-terminal recombinant peptides

Protein Science : a Publication of the Protein Society
M A JiménezOctavio Monasterio

Abstract

We have investigated the solution conformation of the functionally relevant C-terminal extremes of alpha- and beta-tubulin, employing the model recombinant peptides RL52alpha3 and RL33beta6, which correspond to the amino acid sequences 404-451(end) and 394-445(end) of the main vertebrate isotypes of alpha- and beta-tubulin, respectively, and synthetic peptides with the alpha-tubulin(430-443) and beta-tubulin(412-431) internal sequences. Alpha(404-451) and beta(394-445) are monomeric in neutral aqueous solution (as indicated by sedimentation equilibrium), and have circular dichroism (CD) spectra characteristic of nearly disordered conformation, consistent with low scores in peptide helicity prediction. Limited proteolysis of beta(394-445) with subtilisin, instead of giving extensive degradation, resulted in main cleavages at positions Thr409-Glu410 and Tyr422-Gln423-Gln424, defining the proteolysis resistant segment 410-422, which corresponds to the central part of the predicted beta-tubulin C-terminal helix. Both recombinant peptides inhibited microtubule assembly, probably due to sequestration of the microtubule stabilizing associated proteins. Trifluoroethanol (TFE)-induced markedly helical CD spectra in alpha(404-451) and be...Continue Reading

References

Feb 8, 1977·Biochemistry·F Solomon
Nov 1, 1991·The Journal of Cell Biology·K A Butner, M W Kirschner
Jan 5, 1990·Science·B EddéP Denoulet
May 24, 1991·Science·A LupasJ Stock
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
May 5, 1988·Journal of Molecular Biology·H J DysonR A Lerner
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U Z LittauerH Ponstingl
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jan 1, 1986·Methods in Enzymology·J T YangH M Martinez
Oct 1, 1983·Molecular and Cellular Biology·N J CowanD W Cleveland
Jan 1, 1995·Cell Motility and the Cytoskeleton·J M de PeredaJ M Andreu
Jul 1, 1995·The Biochemical Journal·L M Fleming, G V Johnson
Jun 1, 1994·Nature Structural Biology·V Muñoz, L Serrano
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·B BergerP S Kim
Jan 1, 1994·Methods in Enzymology·D S Wishart, B D Sykes
Aug 16, 1994·Biochemistry·N GustkeE Mandelkow
Jan 1, 1993·Cell Motility and the Cytoskeleton·S LobertJ J Correia
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Jan 1, 1997·Cell Motility and the Cytoskeleton·E T O'BrienH P Erickson
Dec 31, 1997·International Review of Cytology·R F Ludueña

❮ Previous
Next ❯

Citations

Apr 8, 2000·Current Opinion in Structural Biology·L A Amos
Nov 11, 2010·The Journal of Biological Chemistry·Julien LefèvrePhilippe Savarin
Dec 17, 2008·The Journal of Biological Chemistry·Patrick A JoeRichard F Ludueña
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Vladimir N Uversky
Aug 1, 2016·Chaos·Dalibor L SekulicMiljko V Sataric
Aug 20, 2016·ACS Chemical Biology·Kathryn P WallLoren E Hough
Mar 5, 2009·European Biophysics Journal : EBJ·M V SatarićJack Adam Tuszynski
Jul 17, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·M KatragaddaA D Albert
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Stefan Westermann, Klaus Weber
Feb 3, 2006·Journal of Muscle Research and Cell Motility·Stefan Lakämper, Edgar Meyhöfer
Aug 5, 2011·Journal of Cellular and Molecular Medicine·Hironori NakagamiYasufumi Kaneda
Jun 6, 2020·Analytical Biochemistry·Colleen McClungCristian I Ruse
Nov 1, 2002·Biochimica Et Biophysica Acta·Arlene D Albert, Philip L Yeagle
Aug 16, 2005·Biophysical Journal·Stefan Lakämper, Edgar Meyhöfer
Jan 16, 2007·Biochimica Et Biophysica Acta·Philip L YeagleAnthony Watts

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.