Helicobacter hepaticus infection in BALB/c mice abolishes subunit-vaccine-induced protection against M. tuberculosis

Vaccine
Isabelle ArnoldElma Z Tchilian

Abstract

BCG, the only licensed vaccine against tuberculosis (TB), provides geographically variable protection, an effect ascribed to exposure to environmental mycobacteria (EM). Here we show that altering the intestinal microbiota of mice by early-life infection with the commensal bacterium Helicobacter hepaticus (Hh) increases their susceptibility to challenge with Mycobacterium tuberculosis (Mtb). Furthermore Hh-infected mice immunised parenterally with the recombinant subunit vaccine, human adenovirus type 5 expressing the immunodominant antigen 85A of Mtb (Ad85A), display a reduced lung immune response and protection against Mtb challenge is also reduced. Expression of interleukin 10 (IL10) messenger RNA is increased in the colon of Hh infected mice. Treatment of Hh-infected Ad85A-immunised mice with anti-IL10 receptor antibody, following challenge with Mtb, restores the protective effect of the vaccine. These data show for the first time that alteration of the intestinal microbiota by addition of a single commensal organism can profoundly influence protection induced by a TB subunit vaccine via an IL10-dependent mechanism, a result with implications for the deployment of such vaccines in the field.

References

May 10, 2001·Scandinavian Journal of Infectious Diseases·P E Fine
Aug 21, 2002·The Journal of Experimental Medicine·Marika C KullbergAlan Sher
Jan 8, 2003·The Journal of Experimental Medicine·Kevin J MaloyFiona Powrie
Mar 23, 2005·Transactions of the Royal Society of Tropical Medicine and Hygiene·D EliasS Britton
Feb 12, 2010·Mucosal Immunology·I I Ivanov, D R Littman
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·June L Round, Sarkis K Mazmanian
Jul 9, 2011·The Journal of Clinical Investigation·Isabelle C ArnoldAnne Müller
Mar 28, 2012·Tuberculosis·Michael J Brennan, Jelle Thole
May 15, 2012·Nature Reviews. Microbiology·Christian LienhardtMario Raviglione
Jan 30, 2013·Experimental Animals·Nobuhito HayashimotoNaoko Kagiyama
Mar 7, 2013·Mucosal Immunology·P J MorrisonM C Kullberg
Nov 21, 2013·Mucosal Immunology·P C L BeverleyE Z Tchilian
Jul 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Daniela B EnglerAnne Müller

❮ Previous
Next ❯

Citations

Sep 16, 2015·Helicobacter·Bram FlahouKeigo Shibayama
May 6, 2016·Frontiers in Cellular and Infection Microbiology·Joshua E DennyNathan W Schmidt
Sep 18, 2016·FEBS Letters·Matthias Hauptmann, Ulrich E Schaible
Feb 18, 2017·Scientific Reports·Gandharva NagpalGajendra P S Raghava
May 29, 2018·Journal of Internal Medicine·C LangeT Schön
Jan 29, 2020·Annals of the New York Academy of Sciences·John Osei SekyerePetrus B Fourie
Nov 16, 2019·Cellular and Molecular Life Sciences : CMLS·Osagie A EriboNovel N Chegou
Jul 19, 2017·The American Journal of Tropical Medicine and Hygiene·Madeleine R WoodSaurabh Mehta
Oct 13, 2020·PloS One·Claudia SalaStewart T Cole
Mar 21, 2017·Veterinary Microbiology·Megan C Niederwerder
May 28, 2021·European Respiratory Review : an Official Journal of the European Respiratory Society·Irina KontsevayaJan Heyckendorf
Dec 6, 2017·ACS Infectious Diseases·Abraham J Waldman, Emily P Balskus

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

FlowJo

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.