Helicobacter pylori and H2O2 increase AP endonuclease-1/redox factor-1 expression in human gastric epithelial cells

Gastroenterology
Song-Ze DingSheila E Crowe

Abstract

Helicobacter pylori infection causes inflammation, accumulation of reactive oxygen species, and oxidative DNA damage in the gastric mucosa. Apurinic/apyrimidinic endonuclease-1 (APE-1)/redox factor-1 (Ref-1) repairs damaged DNA and reductively activates transcription factors, including activator protein-1. Considering that H. pylori generate reactive oxygen species and that reactive oxygen species modulate APE-1/Ref-1 in other cell types, we examined the effect of H. pylori, oxidative stress, and antioxidants on APE-1/Ref-1 expression in human gastric epithelial cells. Human gastric epithelial cell lines or cells isolated from mucosal biopsy samples were stimulated with H. pylori, Campylobacter jejuni, and/or H 2 O 2 in the presence or absence of antioxidants. APE-1/Ref-1 expression was assayed by Western blot or reverse-transcription polymerase chain reaction, and its cellular distribution was determined by using indirect conventional and confocal immunofluorescence. New protein synthesis was detected by [S 35 ]methionine labeling. APE-1/Ref-1 function was assessed by using a luciferase-linked reporter construct containing 3 activator protein 1 binding sites. APE-1/Ref-1 protein and messenger RNA were detected in resting gastr...Continue Reading

Citations

Jun 21, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kimio KawasakiTsutomu Chiba
Sep 11, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Asima BhattacharyyaSheila E Crowe
Apr 3, 2014·Physiological Reviews·Asima BhattacharyyaSheila E Crowe
Jul 10, 2007·The American Journal of Gastroenterology·Hassan AshktorabDuane T Smoot
Jun 8, 2013·American Journal of Respiratory Cell and Molecular Biology·Huang HuangMin Wu
Jan 28, 2014·PloS One·Serkalem TadesseErrol R Norwitz
Aug 21, 2015·World Journal of Gastroenterology : WJG·Juliana Carvalho Santos, Marcelo Lima Ribeiro
Aug 22, 2017·NPJ Precision Oncology·Fenil ShahMark R Kelley
Jun 18, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Richard M Peek
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ann M O'HaraSheila E Crowe
Sep 24, 2005·Helicobacter·Céu FigueiredoYoshio Yamaoka
Nov 23, 2006·International Journal of Hematology·Hidekazu SuzukiToshifumi Hibi
May 4, 2011·Virchows Archiv : an International Journal of Pathology·Isabelle Joyce de Lima Silva-FernandesSilvia Helena Barem Rabenhorst
May 9, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Jyh-Chin YangJohn Y Kao
Sep 29, 2011·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Masashi MiyazakiYutaka Kiyohara
May 19, 2012·TheScientificWorldJournal·Petra Hudler
Sep 21, 2017·Mutagenesis·Isabelle Joyce de Lima Silva-FernandesSilvia Helena Barem Rabenhorst
Apr 27, 2018·Frontiers in Microbiology·Ayse Z SahanSoumita Das
Sep 27, 2018·International Journal of Molecular Sciences·Dawit Kidane
Nov 28, 2007·Annual Review of Pathology·John C Atherton
Feb 4, 2014·Applied and Environmental Microbiology·Rosanne HertzbergerM Joost Teixeira de Mattos
Feb 11, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Soumita DasPeter B Ernst
Dec 2, 2005·Current Opinion in Gastroenterology·Dawn A Israel, Richard M Peek
Mar 1, 2012·Journal of Surgical Oncology·Isabelle Joyce de Lima Silva-FernandesSilvia Helena Barem Rabenhorst
Apr 23, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ana Manuel Dantas MachadoLene Juel Rasmussen
Dec 20, 2020·Microorganisms·Eleftherios KontizasDionyssios N Sgouras
Jan 13, 2006·Gastroenterology·Peter B ErnstSheila E Crowe
May 19, 2007·Pathology, Research and Practice·Sabine KruegerAlbert Roessner
Sep 20, 2015·Gastroenterology·Manuel Amieva, Richard M Peek
Feb 4, 2010·Biochimica Et Biophysica Acta·Ana Manuel Dantas MachadoLene Juel Rasmussen

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Campylobacteriosis

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.