Helicobacter pylori cholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages

Cellular Microbiology
Chih-Ho LaiWen-Ching Wang

Abstract

Cholesterol-α-glucosyltransferase (CGT) encoded by the type 1 capsular polysaccharide biosynthesis protein J (capJ) gene of Helicobacter pylori converts cellular cholesterol into cholesteryl glucosides. H. pylori infection induces autophagy that may increase bacterial survival in epithelial cells. However, the role of H. pylori CGT that exploits lipid rafts in interfering with autophagy for bacterial survival in macrophages has not been investigated. Here, we show that wild-type H. pylori carrying CGT modulates cholesterol to trigger autophagy and restrain autophagosome fusion with lysosomes, permitting a significantly higher bacterial burden in macrophages than that in a capJ-knockout (∆CapJ) mutant. Knockdown of autophagy-related protein 12 impairs autophagosome maturation and decreases the survival of internalised H. pylori in macrophages. These results demonstrate that CGT plays a crucial role in the manipulation of the autophagy process to impair macrophage clearance of H. pylori.

References

Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A P GobertK T Wilson
May 7, 2002·Clinical Medicine : Journal of the Royal College of Physicians of London·Barry Marshall
Mar 28, 2003·Biochemical and Biophysical Research Communications·Chun-Hsien Kuo, Wen-Ching Wang
May 10, 2003·FEMS Immunology and Medical Microbiology·Andreas Munk Petersen, Karen Angeliki Krogfelt
Sep 17, 2004·Nature Reviews. Microbiology·Denise M MonackStanley Falkow
Nov 6, 2004·Science·Ichiro NakagawaTamotsu Yoshimori
Sep 27, 2005·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Mehmet KaplanMahmut Murat Demirtas
Jan 7, 2006·The Journal of Antimicrobial Chemotherapy·Chih-Ho LaiWen-Ching Wang
Feb 24, 2006·The Journal of Biological Chemistry·Cheryl L BirminghamJohn H Brumell
Jul 18, 2006·The Journal of Biological Chemistry·Anne-Helene LebrunDirk Warnecke
Sep 5, 2006·Nature Medicine·Christian WunderThomas F Meyer
Dec 1, 2006·The Journal of Biological Chemistry·Annabelle SchnaithOleg Krut
Mar 29, 2007·Cellular Microbiology·Andre Dubois, Thomas Borén
Nov 6, 2007·Cellular Microbiology·Hua NiuYasuko Rikihisa
Nov 8, 2007·The Journal of Biological Chemistry·Takao HanadaYoshinori Ohsumi
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Dec 10, 2008·Experimental Biology and Medicine·Ya-Hui WangHuan-Yao Lei
Mar 10, 2009·Autophagy·Ya-Hui WangHuan-Yao Lei
Jun 17, 2009·Cell Host & Microbe·Vojo Deretic, Beth Levine
Oct 12, 2010·Clinical Microbiology Reviews·Lydia E WroblewskiKeith T Wilson
Jan 21, 2011·Nature·Beth LevineHerbert W Virgin
Feb 12, 2011·Cell Death and Differentiation·R KangD Tang
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marisa BenagianoMario M D'Elios
Sep 12, 2012·Autophagy·Daniel J KlionskyBrian Zuckerbraun
Oct 26, 2013·Trends in Microbiology·Laura K Greenfield, Nicola L Jones
Jul 30, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Shin-Yi DuWen-Ching Wang

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Citations

Oct 17, 2019·Journal of Lipid Research·Michael I BukrinskyDmitri Sviridov
Jan 17, 2019·Journal of Clinical Medicine·Yi-Hsing ChenChih-Ho Lai
Aug 6, 2020·Particle and Fibre Toxicology·Yu-Wen ChenChih-Ho Lai
Dec 29, 2020·Helicobacter·Majjid A QariaNiyaz Ahmed
Feb 9, 2020·Seminars in Cell & Developmental Biology·Wei Yang SitWen-Ching Wang
Feb 12, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huan HeDapeng Yan

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