Abstract
Helicobacter pylori infection is recognized as the major cause of gastritis and gastric cancer; however, its role in the development of gastroesophageal reflux disease and Barrett's adenocarcinoma is unclear. The expression of NF-kappaB, AP-1, and COX-2 may be important in inflammation and tumorigenesis in the esophagus. The aim of this study was to examine the effect of live H pylori or H pylori extract (HPE) on these factors in the esophageal epithelial cell lines SKGT-4 and OE33. NF-kappaB and AP-1 activity were assessed by gel shift assay and COX-2 by Western blotting. Coculture of SKGT-4 and OE33 with live H pylori and HPE induced NF-kappaB and AP-1 DNA-binding activity, and also decreased IkappaB-alpha levels. Treatment with the specific MEK1/2 MAPK inhibitor PD98059, but not the p38 MAPK inhibitor SB203580, inhibited NF-kappaB and AP-1 activity. The antioxidant vitamin C inhibited H pylori-induced NF-kappaB activation, but increased AP-1 expression. Moreover, HPE induced COX-2 expression and IL-8 production, and PD98059 inhibited COX-2 expression, ERK1/2 phosphorylation, and IL-8 production. These data demonstrate that both live H pylori and HPE induce NF-kappaB and AP-1 expression in esophageal epithelial cells. The ind...Continue Reading
Citations
Sep 18, 2009·BMC Cancer·Showket HussainBhudev C Das
Sep 11, 2012·Journal of Clinical Biochemistry and Nutrition·Jeong-Sang LeeKi-Baik Hahm
Nov 7, 2012·Clinical and Experimental Medicine·Yen-Chun PengLan-Ru Huang
Jun 3, 2014·International Journal of Molecular Sciences·Yuling ZhangHao Zhang
Sep 11, 2014·Journal of the Chinese Medical Association : JCMA·Yen-Chun PengShu-Peng Ho
May 15, 2012·Progress in Lipid Research·Inês Cebola, Miguel A Peinado
May 10, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Limas KupcinskasTorkel Wadström
Jan 2, 2007·Medical Hypotheses·Jannis KountourasChristos Zavos
May 15, 2009·Journal of Cellular and Molecular Medicine·Arun K MankanRoss McManus
May 15, 2013·Microbiology and Immunology·Jung-Min KimSeung-Chul Baik
Jan 19, 2011·Helicobacter·Fang-Xun LiuPei-Pei Gao
Nov 5, 2011·Cellular Microbiology·Gaëlle Le Negrate
Aug 6, 2014·Vaccine·Xiaobo LvTao Xi
Feb 21, 2009·Orvosi hetilap·Krisztina Hagymási, Zsolt Tulassay
Apr 14, 2015·Pediatric Gastroenterology, Hepatology & Nutrition·Zhang BinYvan Vandenplas
Jun 21, 2008·Journal of Periodontal Research·A KishimotoH Kurihara
Dec 25, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Soon Ok ChoHyeyoung Kim