Helicobacter pylori FlgR is an enhancer-independent activator of sigma54-RNA polymerase holoenzyme

Journal of Bacteriology
Priyanka BrahmacharyTimothy R Hoover

Abstract

Helicobacter pylori FlgR activates transcription with sigma54-RNA polymerase holoenzyme (sigma54-holoenzyme) from at least five flagellar operons. Activators of sigma54-holoenzyme generally bind enhancer sequences located >70 bp upstream of the promoter and contact sigma54-holoenzyme bound at the promoter through DNA looping to activate transcription. H. pylori FlgR lacks the carboxy-terminal DNA-binding domain present in most sigma54-dependent activators. As little as 42 bp of DNA upstream of the flaB promoter and 26 bp of DNA sequence downstream of the transcriptional start site were sufficient for efficient FlgR-mediated expression from a flaB'-'xylE reporter gene in H. pylori, indicating that FlgR does not use an enhancer to activate transcription. Other examples of sigma54-dependent activators that lack a DNA-binding domain include Chlamydia trachomatis CtcC and activators from the other Chlamydia spp. whose genomes have been sequenced. FlgR from Helicobacter hepaticus and Campylobacter jejuni, which are closely related to H. pylori, appear to have carboxy-terminal DNA-binding domains, suggesting that the loss of the DNA-binding domain from H. pylori FlgR occurred after the divergence of these bacterial species. Removal of...Continue Reading

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Citations

Dec 1, 2011·Proceedings of the National Academy of Sciences of the United States of America·Joseph M Boll, David R Hendrixson
Dec 25, 2007·Applied and Environmental Microbiology·John C WilliamsTraci L Testerman
May 1, 2012·Journal of Bacteriology·Ashley M GroshongJon S Blevins
Oct 22, 2008·Journal of Bacteriology·Francois P DouillardPaul W O'Toole
Jun 22, 2005·Journal of Bacteriology·Lara Pereira, Timothy R Hoover
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Matthew Bush, Ray Dixon
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jeremy J GilbreathDavid R Hendrixson
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Jun 11, 2005·FEMS Microbiology Letters·Kieran A RyanPaul W O'Toole
Aug 21, 2019·Journal of Bacteriology·Joshua K ChuTimothy R Hoover

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