Helicobacter pylori heat shock protein B (HspB) localizes in vivo in the gastric mucosa and MALT lymphoma

Journal of Cellular Physiology
Antonio De LucaG Iaquinto

Abstract

Heat shock protein B (HspB) is one of the dominant proteins recognized by most Helicobacter pylori-infected persons and is being considered as potential candidates for subunit vaccines. In the present study we describe the generation of an antibody against HspB and its use in immunohistochemical assays on gastric biopsies. We have demonstrated that our rabbit polyclonal antibody against HspB did not recognize any protein in lysates from a lung human epithelial cell (H1299) line and did not cross-react with the other members of human heat shock proteins. Secondly, we have observed that in gastric biopsies, HspB immunostaining was present inside the cytoplasm of human epithelial cells with a particular localization in the apical portion of gastric epithelial cells other than in the extracellular spaces among gastric cells of human stomach. Finally, we have demonstrated a cytoplasmic HspB immunostaining in groups of neoplastic cells of MALT lymphoma. In conclusion, our observations suggest a possible involvement of HspB in the pathogenesis of H. pylori-related pathologies such as gastritis, ulcer and gastric cancer.

References

Oct 17, 1991·The New England Journal of Medicine·J ParsonnetR K Sibley
Oct 17, 1991·The New England Journal of Medicine·A NomuraM J Blaser
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·R L FerreroA Labigne
Feb 7, 1994·NIH Consensus Statement
Jul 1, 1994·Clinical and Experimental Immunology·S A SharmaM J Blaser
Apr 1, 1994·Infection and Immunity·M A TufanoP Marinelli
May 5, 1994·The New England Journal of Medicine·J ParsonnetG D Friedman
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S CensiniA Covacci
Aug 1, 1997·Nature Medicine·C LöfmanL Engstrand
Jul 1, 1997·Gut·M J Farthing
Aug 13, 1998·Alimentary Pharmacology & Therapeutics·E J Kuipers
Mar 20, 1999·Gut·F Stellaard, B Geypens
Jul 30, 1999·Carcinogenesis·P VineisA S Costantini
Oct 12, 2000·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·N PapadogiannakisA Gad
Oct 13, 2000·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·G IaquintoN Figura
May 25, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A De LucaM G Paggi
Oct 9, 2002·Cellular Microbiology·Manuel R AmievaStanley Falkow
Jan 25, 2003·Journal of Internal Medicine·B BjörkholmO Nyrén
May 20, 2004·International Journal of Medical Microbiology : IJMM·Wolfgang FischerRainer Haas
Aug 18, 2004·Cancer Letters·Antonio De Luca, Gaetano Iaquinto
Nov 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Antonio De LucaAlfonso Baldi
Nov 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Fabio BagnoliManuel R Amieva
Jan 24, 2007·Journal of Cellular Physiology·Paraskevi VogiatziAntonio Giordano
May 9, 2007·Journal of Cellular Biochemistry·Paraskevi VogiatziAntonio Giordano

❮ Previous
Next ❯

Citations

Sep 12, 2008·Helicobacter·António Carlos FerreiraYoshio Yamaoka
Feb 3, 2015·Journal of Cellular Physiology·Maria De FalcoAntonio De Luca
Dec 8, 2016·Journal of Cellular Physiology·Giuseppe MazzarellaAntonio De Luca
Dec 19, 2020·Open Medicine·Armando CalogeroMichele Santangelo
Nov 5, 2020·Molecular Therapy Oncolytics·Ianko D IankovEvanthia Galanis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.