PMID: 9187877May 1, 1997Paper

Helicobacter pylori-human polymorphonuclear leucocyte interaction in the presence of ammonia

European Journal of Gastroenterology & Hepatology
K MayoF Mégraud

Abstract

To determine if the ammonia produced by Helicobacter pylori affects the phagocytic ability of human polymorphonuclear leucocytes as measured by the oxidative burst. Interactions between opsonized urease-positive and -negative strains of H. pylori with polymorphonuclear leucocytes were studied in two series of experiments. In the first series of experiments, concentrations from 0 to 50 mM of NH4Cl were added to polymorphonuclear leucocytes. In the second series of experiments, bacteria were pre-incubated for 1 h with urea (0 to 50 mM) before addition of phagocytes. Luminol-dependent chemiluminescence was measured every 5 min over a 50-min period. The pH was verified in each treatment. Inhibition of chemiluminescence, increasing with concentration, was noted in all treatments when NH4Cl was added. When urea was added to urease-positive strains, chemiluminescence was significantly reduced when compared to the urease-negative strain and the zymosan control. This effect could not be attributed to a change in pH in the experiments using NH4Cl or urea at a concentration of 5 mM and 10 mM. Ammonia generated by H. pylori may contribute to the decreased activity of polymorphonuclear leucocytes in vivo.

Citations

Nov 13, 2012·PloS One·Dhammika H M L P NavarathnaDavid D Roberts
Aug 13, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Debbie L ShawcrossRajiv Jalan
Nov 6, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Debbie L ShawcrossRobin D Hughes
Apr 20, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·V HofmanP Hofman
Feb 3, 2005·The Korean Journal of Internal Medicine·Ok-Jae LeeHyun-Jin Kim

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