Helicobacter pylori infection impairs gastric epithelial barrier function via microRNA‑100‑mediated mTOR signaling inhibition

Molecular Medicine Reports
Guimei HuGuoliang Ye

Abstract

Helicobacter pylori (H. pylori) infection has an important effect on human health as it is an established cause of gastric carcinoma. microRNAs (miRNAs/miRs) are a family of small RNAs with various functions in the control of cellular profiles. However, the effect of miR‑100 in H. pylori infection remains unknown. Healthy volunteers (n=100) and patients with H. pylori infection (n=98) were included in the present study. H. pylori infection was confirmed by urea breath tests. The levels of miR‑100 in gastroscopic biopsy samples and cultured GES‑1 cells were measured by reverse transcription‑quantitative polymerase chain reaction. Furthermore, miR‑100 was overexpressed or inhibited in GES‑1 cells by an miR‑100 mimic or inhibitor, respectively. The expression of cell‑junction proteins and members of the mechanistic target of rapamycin kinase (mTOR) signaling pathway was investigated by western blotting. The results demonstrated that miR‑100 levels were upregulated in infected patients and cultured gastric epithelial cells, compared with the respective controls. Additionally, the expression of epithelial (E)‑cadherin and zona occludens‑1 in the gastric mucosa of infected patients and GES‑1 cells was downregulated. Furthermore, infe...Continue Reading

References

Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S CensiniA Covacci
Oct 13, 2000·Gastroenterology Clinics of North America·J E Everhart
Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Jan 15, 2002·The Journal of Biological Chemistry·Matthias SelbachSteffen Backert
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aime T FrancoRichard M Peek
Sep 15, 2007·Biochimica Et Biophysica Acta·Andrea Hartsock, W James Nelson
Aug 1, 2008·American Journal of Physiology. Cell Physiology·Tadayuki OshimaTakashi Joh
May 9, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Bettina Temmesfeld-WollbrückStefan Hippenstiel
Jul 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin HouXuetao Cao
Sep 19, 2009·Nature Reviews. Genetics·Carlo M Croce
Jan 19, 2010·Molecular Endocrinology·Ankur K NagarajaMartin M Matzuk
Mar 23, 2010·Cell·Sergei I GrivennikovMichael Karin
Apr 30, 2010·The New England Journal of Medicine·Kenneth E L McColl
Jun 4, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Bao Hua LiXing Xie
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Prashant JainSteven R Blanke
Mar 1, 2012·Trends in Microbiology·Patrice Boquet, Vittorio Ricci
May 12, 2012·The Lancet Oncology·Catherine de MartelMartyn Plummer
Jul 17, 2012·Microbes and Infection·Joachim Rassow, Michael Meinecke
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Samuel L PalframanKipros Gabriel
Dec 4, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Leesa L SampsonYi Zheng
Jun 2, 2017·Nature Reviews. Disease Primers·Jaffer A AjaniShumei Song

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.